Auxin transport in mutants of Arabidopsis thaliana
The active cell-to-cell transport of the plant hormone auxin is directional. We investigate the effect of mutations in hormone signal transduction pathways on polar auxin transport in Arabidopsis thaliana roots (Figure 1).
3-[5(n)- 3H] Indolylacetic acid is fed to root tips in a line of agar. Root segments are harvested after 5 h and the amount of transported auxin is determined using a scintillation counter.
The auxin transport inhibitor NPA is used as a control (Figure 2).
Figure 1: Arabidopsis thaliana on agar plates
Figure 2: Effect of the auxin transport inhibitor NPA