Auxin transport in mutants of Arabidopsis thaliana

The active cell-to-cell transport of the plant hormone auxin is directional. We investigate the effect of mutations in hormone signal transduction pathways on polar auxin transport in Arabidopsis thaliana roots (Figure 1).

3-[5(n)- 3H] Indolylacetic acid is fed to root tips in a line of agar. Root segments are harvested after 5 h and the amount of transported auxin is determined using a scintillation counter.

The auxin transport inhibitor NPA is used as a control (Figure 2).





Projekt21
Figure 1: Arabidopsis thaliana on agar plates





Projekt22
Figure 2: Effect of the auxin transport inhibitor NPA












LARI-Logo


Anschrift:
Labor für Radioisotope
Büsgenweg 2
37077 Göttingen
(Wegbeschreibung)

Leitung:
Prof. Dr. A. Polle


Ansprechpartner:
Bernd Kopka
Tel. +49 (0)551 / 39-8115 (Büro)
Tel. +49 (0)179 / 4515958 (Mobil)
Fax +49 (0)551 / 39-22705
bkopka@gwdg.de





Antragsformular-Icon-klein
Antragsformular
LARI-Nutzung