Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und Molekulare Biowissenschaften

Urlaub, Henning, Prof. Dr.

Group Leader - Bioanalytical Mass Spectrometry Group

  • from 2010: Group leader “Bioanalytical Mass Spectrometry” group at the Max Planck Institute for Biophysical Chemistry, Göttingen and “Bioanalytics” group at University Medical Center Göttingen (UMG) within Dept. of Clinical Chemistry
  • 2010: Professor at the Faculty of Medicine at Georg August University Göttingen
  • 2005: Research group “Bioanalytical Mass Spectrometry Group” at the Max Planck Institute for Biophysical Chemistry
  • 2001: Responsibility for running the mass spectrometry unit in the Dept. of Cellular Biochemistry at the Max Planck Institute for Biophysical Chemistry in Göttingen
  • 2000–2001: Guest researcher at the EMBL in Heidelberg, Germany, in the group of Dr. Matthias Wilm
  • 1997–2001: Post-Doc at the "Institut für Molekularbiologie und Tumorforschung" (IMT) of the Philipps University of Marburg, Germany (Group of Reinhard Lührmann) and at the Max Planck Institute for Biophysical Chemistry in Göttingen (Group of Reinhard Lührmann)
  • 1993–1996 Ph.D. and Post-Doc in the research group of Prof. Brigitte Wittmann-Liebold at the Max Delbrück Center for Molecular Medicine (MDC) in Berlin
  • 1992–1993 Diploma thesis in the research group of Prof. Volker A. Erdmann at the Institute of Biochemistry of the Free University of Berlin
  • 1987–1993 Studied biochemistry at the Free University of Berlin, Germany


  • Major Research Interests

    Modern mass-spectrometric methods have become key technologies in the life sciences. We apply “state-of-the-art” mass spectrometry to elucidate quantitative changes of proteins and their post-translational modifications derived from different samples, including tissue, cells, organelles, and cell compartments. In addition we apply mass spectrometric methods to monitor dynamic changes of protein and protein-ligand complexes through use of crosslinking and chemical probing. In this respect, we collaborate with several groups within the GGNB, like the groups of Wolfgang Fischle, Dirk Görlich, Reinhard Jahn, Reinhard Lührmann, Peter Rehling, Oliver Schlüter, Holger Stark, Jürgen Wienands, Markus Zweckstetter, and many others. We provide solutions and analytical workflows for solving cell biological issues; we further develop novel analytical workflows for in-depth analyses of entire proteomes and for structural analyses of proteins.


    Homepage Department/Research Group

    http://www.mpibpc.mpg.de/urlaub



    Selected Recent Publications

  • Schmitzová J, Rasche N, Dybkov O, Kramer K, Fabrizio P, Urlaub H, Lührmann R, Pena V (2012) Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein. EMBO J 31(9):2222-34
  • Nikolov M, Stuetzer A, Mosch K, Krasauskas A, Soeroes S, Stark H, Urlaub H, Fischle W (2011) Chromatin affinity purification and quantitative mass spectrometry defining the interactome of histone modification patterns. Mol Cell Proteomics10(11):M110.005371 *co-corresponding author
  • Oellerich T, Bremes V, Neumann K, Bohnenberger H, Dittmann K, Hsiao H-H, Engelke M, Schnyder T, Batista F, Urlaub H*, Wienands J (2011) The B cell antigen receptor signal through a preformed transducer module of SLP65 and CIN85. EMBO J 30(17):3620-34 *co-corresponding author
  • Kramer K, Hummel P, Hsiao HH, Luo X, Wahl M, Urlaub H (2011) Mass-spectrometric analysis of proteins cross-linked to 4-thio-uracil- and 5-bromo-uracil-substituted RNA. Int J Mass Spec 304: 184-194
  • Schmidt C, Lenz C, Grote M, Lührmann R, Urlaub H (2010) Determination of protein stoichiometry within protein complexes using absolute quantification and multiple reaction monitoring. Anal Chem 82: 2784-2796
  • Gronborg M, Pavlos N, Brunk I, Chua J, Münster-Wandowski A, Riedel D, Ahnert-Hilger G, Urlaub H*, Jahn R (2010) Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein. J Neurosci 30: 2-12 * co-corresponding author
  • Richter FM, Sander B, Golas MM, Stark H, Urlaub H (2010) Merging molecular electron microscopy and mass spectrometry by carbon film-assisted endoproteinase digestion. Mol Cell Proteomics 9: 1729-41
  • Kühn-Hölsken E, Lenz C, Dickmanns A, Hsiao HH, Richter FM, Kastner B, Ficner R, Urlaub H (2010) Mapping the binding site of snurportin 1 on native U1 snRNP by cross-linking and mass spectrometry. Nucleic Acids Res 38: 5581-93
  • Hsiao HH, Meulmeester E, Frank BT, Melchior F, Urlaub H (2009) "ChopNspice", a mass-spectrometric approach that allows identification of endogenous SUMO-conjugated peptides. Mol Cell Proteomics 8: 2664-2675
  • Oellerich T, Grønborg M, Neumann K, Hsiao HH, Urlaub H*, Wienands J (2009) SLP-65 phosphorylation dynamics reveals a functional basis for signal integration by receptor-proximal adaptor proteins. Mol Cell Proteomics 8: 1738-1750 *co-corresponding author