Sensory Research in Göttingen

Dr rer. nat. Ellen Reisinger

Junior Research Group “Molecular Biology of Cochlear Neurotransmission”
Dept. of Otolaryngology
- Diploma in Biochemistry at the University of Tübingen, 2000

- PhD degree of the Faculty of Biology, University of Freiburg, 2005

- Postdoctoral research fellow (Scholarship of the Research Training Group 843) at the Department of Physiology II, University of Freiburg, 2005 - 2006

- Postdoctoral research fellow at the Department of Otolaryngology, University of Göttingen, and the Department for Molecular Neurobiology of the Max-Planck-Institute for Experimental Medicine, Göttingen since 2006

- Head of the Molecular Biology of Cochlear Neurotransmission junior research group, University of Göttingen since 2007


Major Research Interests

Our group is interested in the molecular mechanisms of hearing and deafness. In order to understand why a certain missense mutation causes deafness in humans, we study structure and biochemical properties of protein domains which are encoded by so called deafness genes. E.g., we study the binding of protein domains to phospholipids and/or Ca2+ as well as protein-protein interactions. Next, we examine the effect of pathogenic mutations on these interactions. Further, we analyze mRNA stability and expression levels by quantitative PCR. In collaboration with other groups in Göttingen, we study our proteins of interest by CD-spectrometry, X-ray crystallography and isothermal titration calorimetry. Further, we are interested in rescuing the deafness phenotype by genetic manipulation of auditory hair cells. For this, we use virus transduction to transform auditory hair cells of deaf mouse mutants in vivo and in vitro and analyze rescue of function by auditory brainstem response and patch clamp.


Homepage Department/Research Group:

http://www.innerearlab.uni-goettingen.de


Recent Publications

Pangrsic T, Lasarow L, Reuter K, Takago H, Schwander M, Riedel D, Frank T, Tarantino LM, Bailey JS, Strenzke N, Müller U, Brose N, Reisinger E*, Moser T* (2010) Hearing requires otoferlin-dependent efficient replenishment of synaptic vesicles in hair cells. Nature Neurosci 13:869-876 (* sharedcorrespondence)

Reisinger E, Meintrup D, Oliver D, Fakler B (2010) Gene expression associated with the onset of hearing detected by differential display in rat organ of Corti. Eur J Hum Genetics, doi:10.1038/ejhg.2010.111

Kerr MA, Reisinger E, Jonas P (2008) Differential dependence of phasic transmitter release on synaptotagmin 1 at GABAergic and glutamatergic hippocampal synapses. PNAS 105:15581-15586

Aponte Y, Lien CC, Reisinger E, Jonas P (2006) Hyperpolarization-activated cation channels in fast-spiking interneurons of rat hippocampus. J Physiol 574:229-243

Berkefeld H, Sailer CA, Bildl W, Rohde V, Thumfart JO, Eble S, Klugbauer N, Reisinger E, Bischofberger J, Oliver D, Knaus HG, Schulte U, Fakler B (2006) BKCa-Cav channel complexes mediate rapid and localized Ca2+-activated K+ -signaling. Science 314:615-620

Reisinger E, Zimmermann U, Knipper M, Ludwig J, Klöcker N, Fakler B, Oliver D (2005). Cod106, a novel synaptic protein expressed in sensory hair cells of the inner ear and in CNS Neurons. Mol Cell Neurosci 28:106-117

Meintrup D, Reisinger E (2005). A statistical model providing comprehensive predictions for the mRNA differential display. Bioinformatics 21:3880-3886