Group Dr. Adio
Single molecule biochemistry of macromolecular machines
Molecular machines are enzymes that generate directed motion within cells. Their action is required in virtually all vital processes. Prime examples for molecular machines are ribosomes, which read the messenger RNA in order to translate it into proteins. My research centers on the understanding of how ribosomes generate motion along the mRNA and how the internal motions of the ribosome translate into its function in protein synthesis.To this end, my group employs a multidisciplinary approach bridging state-of-the-art single-molecule fluorescence microscopy with classic molecular biology and biochemistry techniques. We use these methods to visualize the multitude of intermolecular movements on individual ribosome complexes as well as the large-distance displacement of single ribosomes on the mRNA. On single molecule level, we address fundamental questions on the mechanism of translation:
- How fast and processive does the ribosome transit along the mRNA?
- How does the ribosome negotiate obstacles imposed by RNA secondary structure or RNA binding proteins?
- How is ribosome motion regulated?
- How does aberrant translation regulation relate to the development of diseases?
My group is building up.
Students interested in Bachelor- and Masterthesis as well as labrotations are welcome.