Dosch, Roland, Dr.
Group Leader at the Dept. of Developmental Biochemistry
- 1994-1999 PhD, Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Germany
- 1999-2003 Postdoc University of Pennsylvania, Philadelphia, USA
- 2004-2010 Junior group leader, University of Geneva, Switzerland
- 2010-2018 Group leader at the Inst. of Developmental Biochemistry, Georg August University, Göttingen
- since 2018 Group leader at the Inst. of Human Genetics, University Medical Center, Göttingen
Major Research Interests
A fundamental principle of biological systems is their capacity to reproduce, which is not found in other domains of science such as chemistry or physics. In multicellular organisms like humans, this unique activity is achieved by gametes, egg and sperm. To prepare for the development of a novel organism after fertilization, the oocyte shows a fascinating organization into various compartments.
The aim of our research is to understand the molecular mechanisms, which control the cellular organization of the oocyte. For our experiments, we take advantage of the zebrafish, which in recent years emerged as an outstanding vertebrate model to investigate molecular processes in vivo. We previously isolated a collection of mutations in key regulators, which show defects in the organization of the oocyte. We apply a combination of molecular genetics and cutting edge genomics such as next-generation-sequencing to identify the affected genes in these mutants. In the most interesting mutants, we started to characterize the molecular function of these essential genes. For this purpose, we incorporate biochemical methods with cell biological approaches e.g. imaging to explore the dynamics of protein localization in vivo. With these techniques, we discovered proteins controlling the assembly of RNA-granules as an example for a membrane-free compartment. Recently, we also analyzed membrane bound compartments and identified an important regulator of secretion. Our long-term goal is to understand the intricate molecular organization of the oocyte, which prepares it for fertilization and subsequent embryogenesis.
Homepage Department/Research Group
- Krishnakumar P, Riemer S, Perera R, Lingner T, Goloborodko A, Khalifa H, Bontems F, Kaufholz F, El-Brolosy MA, Dosch R (2018) Functional equivalence of germ plasm organizers. PLoS Genet in press
- Roovers EF, Kaaij LJT, Redl S, Bronkhorst AW, Wiebrands K, de Jesus Domingues AM, Huang HY, Han CT, Riemer S, Dosch R, Salvenmoser W, Grun D, Butter F, van Oudenaarden A, Ketting RF (2018) Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification. Dev Cell, 46(3), 285-301 e289. doi:10.1016/j.devcel.2018.07.009
- Dosch R, (2015) Next generation mothers: Maternal control of germline development in zebrafish. Crit Rev Biochem Mol Biol 50: 54-68
- Riemer S, Bontems F, Krishnakumar P, Gömann J, Dosch R, (2015) A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish. Gene Expr Patterns 18: 44-52
- Kanagaraj P, Gautier-Stein A, Riedel D, Schomburg C, Cerda J, Vollack N, Dosch R (2014) Souffle/Spastizin controls secretory vesicle maturation during zebrafish oogenesis. PLoS Genet 10: e1004449
- Bontems F, Baerlocher L, Mehenni S, Bahechar I, Farinelli L, Dosch R (2011) Efficient mutation identification in zebrafish by microarray capturing and next generation sequencing. BBRC 405(3): 373-376
- Bontems F, Stein A, Marlow F, Lyautey J, Mullins MC, Dosch R (2009) Bucky ball organizes germ plasm assembly in zebrafish. Curr Biol 19 (5): 414-22