Adio, Sarah, Dr.

GZMB Research Group Leader


  • PhD thesis at the Ludwig-Maximilians University of München, 2003-2007
  • Post-doctoral fellow at the National Institute for Medical Research (NIMR), London, United Kingdom, 2007-2008
  • Post-doctoral researcher at the Max-Planck Institute for biophysical Chemistry Göttingen, 2008-2017
  • Research Group Leader at the Göttingen Center for Molecular Biology (GZMB) of the University of Göttingen, since 2017



Major Research Interests

Molecular machines are enzymes that generate directed motion within cells. Their action is required in virtually all vital processes. Prime examples for molecular machines are ribosomes, which read the messenger RNA in order to translate it into proteins. My research centers on the understanding of how ribosomes generate motion along the mRNA and how the internal motions of the ribosome translate into its function in protein synthesis. To this end, my group employs a multidisciplinary approach bridging state-of-the-art single-molecule fluorescence microscopy with classic molecular biology and biochemistry techniques. Our work aims to visualize the multitude of small-scale intermolecular movements on individual ribosome complexes as well as the large scale displacement of single ribosomes on the mRNA. On single molecule level, we establish methods to address fundamental questions on the mechanisms of ribosome motility:


  • 1. How fast and processive does the ribosome transit along the mRNA
  • 2. How does the ribosome negotiate obstacles imposed by RNA secondary structure or RNA binding proteins?
  • 3. How is ribosome motion regulated?



Homepage Department/Research Group

http://www.uni-goettingen.de/en/579309.html

Selected Recent Publications


  • Dynamics of ribosomes and release factors during translation termination in E.coli
    Adio S.*, Senjushkina T., Sharma H., Karki P., Maracci C., Peske F., Holtkamp W., Rodnina MV.
    eLife. 2018 Jun 11; doi: 10.7554/eLife.34252

  • Kinetics of Spontaneous and EF-G-Accelerated Rotation of Ribosomal Subunits
    H. Sharma, Adio S.*, T. Senyushkina, R. Belardinelli, F. Peske, MV. Rodnina Cell Rep. 2016 Aug 23; doi: 10.1016/j.celrep.2016.07.051

  • Fluctuations between multiple EF-G-induced chimeric tRNA states during translocation on the ribosome
    Adio S.* T. Senjushkina, F. Peske, N. Fischer, W. Wintermeyer, M. Rodnina
    Nat Commun. 2015 Jun 15; doi: 10.1038/ncomms8442
    Dissection of kinesin's processivity

  • Adio S.*, J. Jaud, B. Ebbing, M. Rief, G. Woehlke
    PLoS One. 2009; doi: 10.1371/journal.pone.0004612

  • The human kinesin Kif18A is a motile microtubule depolymerase essential for chromosome congression
    M.I. Mayr, S. Hümmer, J. Bormann, T. Grüner, Adio S.*, G. Woehlke, T.U. Mayer
    Curr Biol. 2007 Mar 20; 17(6):488-98

  • Kinetic and mechanistic basis of the nonprocessive Kinesin-3 motor NcKin3 Adio S.*, M. Bloemink, M. Hartel, S. Leier, M.A. Geeves, G. Woehlke
    J Biol Chem. 2006 Dec 8; 281(49):37782-93