Key competence module: "Methods course: Enzyme catalysis and biological chemistry" (6 C, 10 WLH) [M.Bio.168]

Learning outcome, core skills: Recombinant expression of enzymes and purification by chromatographic methods (gel filtration, affinity and ion exchange chromatography), kinetic characterisation of enzymatic reactions by steady-state assays and transient kinetics (stopped-flow, quench-flow), thermodynamic characterisation of enzyme : inhibitor or enzyme : substrate interactions by spectroscopic methods (circular dichroism, fluorescence spectroscopy, UV-Vis spectroscopy, NMR spectroscopy) as well as microcalorimetric methods (ITC), chemical synthesis of biooligomers and of ligands, synthesis of organic and inorganic (metallo-)complexes.


Courses:
Methods course: "Structural Biochemistry" (10 WLH)

Examination: protocol (max. 10 pages)
Prerequisite for examination: regular participation in methods course, submission of all protocols in time


Selection options
elective-compulsory

Admission requirements
M.Bio.106 or M.Bio.107

Recommended previous knowledge
none

Number of repeat examinations permitted
twice

Module applicable in the following degree programmes
MSc Microbiology and Biochemistry

Course frequency
each winter semester

Duration
1 semester

Language
English

Maximum number of students
5

Workload
180 h (140/40 h, Attendance time/Self-study time)

Person responsible for module
Prof. Dr. Kai Tittmann