Abteilung Nutzpflanzengenetik

Development and utilization of doubled haploids in Brassica napus (Ariana Valdés)

Microspore culture in Brassica napus is applied with the following aims:

  • Study of distorted molecular marker segregation phenomena in DH populations
  • Development of doubled haploid populations for genetic studies
  • Improvement of the regeneration frequency of microspore derived embryos (MDE)
  • Improvement of the speed and regeneration frequency of plants from MDE
  • Improvement of the diploidization of microspores during an early stage of in vitro development
  • Development of in vitro selection for various seed quality traits applying the ‘single cotyledon technique’ (analog to the ‘half seed technique’)


    • Albrecht, S., C. Möllers and G. Röbbelen 1994: Selection for fatty acid composition in microspore-derived embryoids (MDE) of rapeseed, Brassica napus (L.). J. Plant Physiol. 143, 526-529.
    • Albrecht, S., C. Möllers and G. Röbbelen 1995: Selection in vitro for erucic acid content in segregating populations of microspore derived embryoids of Brassica napus. Plant Breeding 114, 210-214.
    • Iqbal, M.C.M, and C. Möllers 2003: Uptake and distribution of sinigrin in microspore derived embryos of Brassica napus L. Journal of Plant Physiol., 8, 961-966.
    • Iqbal, M.C.M., C. Möllers and G. Röbbelen 1994: Increased embryogenesis after colchicine treatment of microspore cultures of B. napus. J. Plant Physiol. 143, 222-226.
    • Iqbal, M.C.M., G. Röbbelen and C. Möllers 1995: Biosynthesis of glucosinolates by microspore-derived embryoids and plantlets in vitro of Brassica napus L.. Plant Science 112, 107-115.
    • Möllers, C. and S. Albrecht 1994: Screening herbicide effects on lipid metabolism of storage lipids by in vitro culture of microspore-derived embryoids of Brassica napus. J. Plant Physiol. 144, 376-384.
    • Möllers, C., 1999: Anwendungen und Potential der Mikrosporenkultur beim Raps. In: Bioengineering für Rapssorten nach Maß. D. Brauer, G. Röbbelen, R. Töpfer (Eds.). Vortr. Pflanzenzüchtg. 45: 29-42.
    • Möllers, C., B. Rücker, D. Stelling and A. Schierholt 2000: Selection in vitro for oleic and linoleic acid content in segregating populations of microspore derived embryos of Brassica napus. Euphytica, 112, 195-201.
    • Möllers, C., L. Nehlin, K. Glimelius and M.C.M. Iqbal 1999: Growth and biosynthesis of glucosinolates in microspore-derived embryos of Brassica napus L.. Physiologia Plantarum 107, 461-446.
    • Möllers, C., M.C.M. Iqbal and G. Röbbelen 1994: Efficient diploidization of Brassica napus by colchicine treatment of microspores and regeneration of doubled haploid plants. Euphytica 75, 95-104.
    • Möllers, C., S. Albrecht and G. Röbbelen 1994: Effect of in vitro culture conditions on fatty acid desaturation in microspore derived embryoids of Brassica napus. J. Plant Physiol. 143, 530-533.
    • Nehlin, L., C. Möllers and K. Glimelius 1995: High frequency of secondary embryogenesis in microspore-derived embryos of Brassica napus L.. Plant Science 111, 219-227.
    • Nehlin, L., C. Möllers, S. Stymne and K. Glimelius 1996: Fatty acid composition in microspore-derived secondary embryos of Brassica napus L.. Plant Science 120, 205-214.
    • Nehlin, L., P. Bergmann, C. Möllers, und K. Glimelius 2000: Transient ß-gus and gfp gene expression and viability analysis of microprojectile bombarded microspores of Brassica napus L.. J. Plant Physiol. 156, 175-183.
    • Nath, U.K., M.C.M. Iqbal, und C. Möllers 2007 : Early, non-destructive selection of microspore derived embryo genotypes in oilseed rape (Brassica napus L.) by molecular markers and oil quality analysis. Mol. Breed. 19, 285–289.
    • Möllers C, Iqbal MCM (2009) Doubled haploids in breeding winter oilseed rape. In: Touraev A, Forster BP, Jain SM (eds.). Advances in Haploid Production in Higher Plants. Springer Science. Pp. 161-170