Jahn, Reinhard, Prof. Dr.
Professor, Director at the Max Planck Institute for Biophysical Chemistry
- Dr. rer. nat., University of Göttingen, Germany, 1981
- Assistant Professor, The Rockefeller University, New York, USA, 1985
- Junior Group Leader, Max Planck Institute for Psychiatry, Martinsried, Germany, 1986
- Associate Professor of Pharmacology and Cell Biology, Yale University, and Investigator, Howard Hughes Medical Institute, New Haven, USA, 1991
- Professor of Pharmacology and Cell Biology, Yale University, New Haven, 1995
- Director of the Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany, 1997
- Adjunct Professor of Pharmacology, Yale University School of Medicine,New Haven, USA 1997-2001
- Adjunct Professor of Biology, University of Göttingen, Germany, 2001
Major Research Interests
Our group is interested in the mechanisms of membrane fusion, with the main emphasis on regulated exocytosis in neurons. Intracellular membrane fusion events are mediated by a set of conserved membrane proteins, termed SNAREs. For fusion to occur, complementary sets of SNAREs need to be present on both of the fusing membranes, which then assemble in a zipper-like fashion to initiate membrane merger. The neuronal SNAREs are among the best characterized. They are the targets of the toxins responsible for botulism and tetanus, and they are regulated by several addtional proteins including synaptotagmin, the calcium sensor for neurotransmitter release. To understand how these proteins mediate fusion, we study their properties in vitro with biochemical and biophysical approaches using native and artificial membranes.
In a second set of projects, we are interested in the mechanisms by which synaptic vesicles sequester and store neurotransmitters. Uptake is mediated by specific vesicular neurotransmitter transporters that are energized by an electrochemical proton gradient across the membrane. Presently we aim for a better understanding of the transport mechanisms using a variety of biochemical and biophysical approaches including imaging of single vesicles. Finally, we use quantitative proteomics to better understand how the presynaptic protein network contributes to the regulation of synaptic release, focusing on protein phosphorylation.
Homepage Department/Research Group
Selected Recent Publications
- Farsi Z, Preobraschenski J, van den Bogaart G, Riedel D, Jahn R*, Woehler A. (2016) Single-vesicle imaging reveals different transport mechanisms between glutamatergic and GABAergic vesicles. Science, 351, 981-984. *corresponding author
- Ryo JK, Min D, Rah SH, Kim SJ, Park Y, Kim H, Kim HM, Jahn R*, Yoon TY* (2015) Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. Science, 347, 1485-1489 (*corresponding authors)
- Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJE, Jahn R (2015) The GTPase Rab26 links synaptic vesicles to the autophagy pathway. eLife 4, e05597
- Par, Y, Seo JB, Fraind A, Perez-Lara A, Yavuz H, Han K, Jung SR, Kattan I, Walla PJ, Choi MY, Cafiso DS, Koh D, Jahn, R (2015) Synaptotagmin-1 binds to PI(4,5)P2-containing membranes but not to SNAREs in a physiological ionic environment. Nature Struct. Mol. Biol., 10, 815-823
- Honigmann A, van den Bogaart G, Iraheta E, Risselada JH, Milovanovic D, Mueller V, Muellar S, Diederichsen U, Fasshauer D, Grubmüller H, Hell SW, Eggeling C, Kühnel K, Jahn R (2013) Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment. Nature Struct Mol Biol 20, 679?686
- Jahn R, Fasshauer D (2012) Exocytosis of synaptic vesicles ? molecular machines, calcium, and beyond (review). Nature, 490(7419):201-7
- Hernandez JM, Stein A, Behrmann E, Riedel D, Cypionka A, Farsi Z, Walla PJ, Raunser S, Jahn R (2012) Membrane fusion intermediates via directional and full assembly of the SNARE complex. Science 336, 1581-1584
- van den Bogaart G, Meyenberg K, Risselada JH, Amin H, Willig KI, Hubrich BE, Dier M, Hell SW, Grubmüller H, Diederichsen U, Jahn R (2011) Membrane protein sequestering by ionic protein-lipid interactions. Nature 479, 552-555