Event
Painting the cell: fluorescent probes for cytoskeleton nanoscopy in living cells| Title of the event | Painting the cell: fluorescent probes for cytoskeleton nanoscopy in living cells |
| Series | MPI Campus Seminar |
| Organizer | Max-Planck-Institut für biophysikalische Chemie (MPIBPC) |
| Speaker | Rūta Gerasimaitė |
| Speaker institution | Research Group Chromatin Labeling and Imaging |
| Type of event | Seminar |
| Category | Forschung |
| Registration required | Nein |
| Details | A large toolbox of fluorescent dyes and proteins enables colouring cellular structures for following their dynamics in living cells or deciphering fine structural details in the fixed samples. Small fluorescent probes come in particularly handy as they are very easy to use and, most importantly, they do not require genetic modification of the cell. These probes are constructed by linking a desired fluorophore to a targeting moiety – a well characterized high affinity drug or inhibitor binding to a particular protein, structure or organelle. Theoretically, this modular design can provide endless palette of probes in all colours of rainbow. In practice, finding an optimal fluorophore, ligand and linker combination can make all the difference between useless and excellent-performing probes. In this talk, I will present probes for cytoskeleton nanoscopy in different colours. I will illustrate how subtle differences in fluorophore and linker structures can have a dramatic impact on probe performance in vivo. |
| Date | Start: 28.04.2021, 11:00 Uhr Ende: 28.04.2021 , 12:00 Uhr |
| Location |
Max-Planck-Institut für biophysikalische Chemie (MPIBPC) (Am Faßberg 11) Online |
| Contact |
05513961108 stefan.gloeggler@mpibpc.mpg.de |