Microsatellite markers (SSRs) isolation for the genus Prosopis and its application to a mating system analysis in a hybrid swarm between Prosopis chilensis and P. flexuosa

The genus Prosopis L. (Fabaceae) constitutes a very important resource in many arid and semiarid regions of the world. In the Argentinean Arid Chaco, Prosopis chilensis and P. flexuosa, two closely related hardwood arboreal species, are important multipurpose species. However, the exploration of the genetic resources of both species is in an early stage. The development of highly polymorphic microsatellite markers (SSRs = Simple Sequence Repeats) is desirable for genetic studies of these species. We develop microsatellite markers for Prosopis chilensis and P. flexuosa using a non-radioactive enrichment strategy for SSR isolation. The markers will be used to observe genetic variation in a natural population of Prosopis chilensis and P. flexuosa of the Argentinean Arid Chaco. Cross-species amplification will be tested in other Prosopis species in order to define the possible cross-species transferability. With the developed markers we will analyze and characterize the mating system of Prosopis chilensis, P. flexuosa and their natural hybrids in a hybrid swarm in the Argentinean Arid Chaco. This research will include studies on gene flow, pollen movement in the hybrid swarm, and on the degree of reproductive compatibility between the mentioned species. These studies will provide valuable information on micro-evolutionary processes that occur within these hybrid swarms.

Members Finkeldey, Reiner (Leiter)
Gailing, Oliver (Mitarbeiter)
Motura, Martin (Mitarbeiter)