Department for Molecular Structural Biology

2017




  • Ficner, R., Dickmanns, A., Neumann, P. (2017). Studying Structure and Function of Spliceosomal Helicases. Methods, Aug 1; 125:63-69 [Abstract]

  • Newie J, Neumann P, Werner M, Mata RA, Ficner R, Feussner I. (2017). Lipoxygenase 2 from Cyanothece sp. controls dioxygen insertion by steric shielding and substrate fixation. Sci Rep. May 18;7(1):2069. [Abstract] PDB: [5MED] PDB: [5MEE] PDB: [5MEF] PDB: [5MEG]

  • Tauchert MJ, Fourmann JB, Lührmann R, Ficner R. (2017). Structural insights into the mechanism of the DEAH-box RNA helicase Prp43. Elife.Jan 16;6 [Abstract] PDB: [5LTA] PDB: [5LTJ] PDB: [5LTK]

  • Schmitt K, Smolinski N, Neumann P, Schmaul S, Hofer-Pretz V, Braus GH, Valerius O. (2017). Asc1p/RACK1 connects ribosomes to eukaryotic phospho-signaling. Mol. Cell. Biol., Jan 19:37(3) [Abstract]

  • Fourmann JB, Tauchert MJ, Ficner R, Fabrizio P, Lührmann R. (2017) Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2. Nucleic Acids Res., Apr 20; 4068 - 4080 [Abstract]


  • 2016




    • Fischer, N., Neumann, P., Bock, LV., Maracci, C., Wang, Z., Paleskava, A., Konevega, AL., Schröder, GF., Grubmüller, H., Ficner, R., Rodnina, MV. and Stark, H. (2016) The pathway to GTPase activation of elongation factor SelB on the ribosome. Nature, 540: 80-85. [Abstract]

    • Boesler, C., Rigo, N., Anokhina, MM., Tauchert, MJ., Agafonov DE., Kastner, B., Urlaub, H., Ficner, R., Will, CL. and Lührmann, R. (2016) A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity. Nat Commun. 7:11997. [Abstract]

    • Tauchert, MJ., Hémonnot, C., Neumann, P., Köster, S., Ficner, R. and Dickmanns A. (2016) Impact of the crystallization condition on importin-? conformation. Acta Crystallogr D Struct Biol. 72:705-717. [Abstract] PDB: [4XRI] [4XRK]

    • Tauchert, MJ. and Ficner, R. (2016) Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum. Acta Crystallogr F Struct Biol Commun. 72:409-16. [Abstract] PDB: [5DTU]

    • Fourmann, JB., Dybkov, O., Agafonov, DE., Tauchert, MJ., Ficner, R., Fabrizio, P. and Lührmann, R. (2016) The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction. Elife. Apr 26;5 [Abstract]

    • Mahal, K., Biersack, B., Schruefer, S., Resch, M.,Ficner, R., Schober, R. and Mueller, T. (2016) Combretastatin A-4 derived 5-(1-methyl-4-phenyl-imidazol-5-yl)indoles with superior cytotoxic and anti-vascular effects on chemoresistant cancer cells and tumors. Eur J Med Chem. 118:9-20. [Abstract]

    • Koch, C., Neumann, P., Valerius, O., Feussner, I. and Ficner, R. (2016) Crystal Structure of Alcohol Oxidase from Pichia pastoris. PLoS One Feb 23;11(2) [Abstract] PDB: [5HSA]

    • Tauchert, MJ., Fourmann, JB., Christian, H., Lührmann, R. and Ficner R. (2016) Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum. Acta Crystallogr F Struct Biol Commun. 72:112-20. [Abstract] PDB: [5D0U]

    • Newie, J., Andreou, A., Neumann, P., Einsle, O., Feussner, I. and Ficner, R. (2016) Crystal structure of a lipoxygenase from Cyanothece sp. may reveal novel features for substrate acquisition. J Lipid Res. 57: 276-87. [Abstract] PDB: [5EK8]


    • 2015





      • Monecke, T. , Dickmanns, A., Weiss, MS., Port, SA., Kehlenbach, RH. and Ficner R. (2015) Combining dehydration, construct optimization and improved data collection to solve the crystal structure of a CRM1-RanGTP-SPN1-Nup214 quaternary nuclear export complex. Acta Crystallogr F Struct Biol Commun. Dec 1;71(Pt 12):1481-1487. [Abstract] PDB: [5DIS]

      • Dickmanns, A. , Kehlenbach, RH. and Fahrenkrog B. (2015) Nuclear Pore Complexes and Nucleocytoplasmic Transport: From Structure to Function to Disease. Int Rev Cell Mol Biol. 320:171-233. [Abstract]

      • Port, SA., Monecke, T., Dickmanns, A., Spillner, C., Hofele, R., Urlaub, H., Ficner, R. and Kehlenbach, RH. (2015)Structural and Functional Characterization of CRM1-Nup214 Interactions Reveals Multiple FG-Binding Sites Involved in Nuclear Export. Cell Rep. Oct 27; 13(4): 690-702 [Abstract] PDB: [5DIS]


      • Wechsler, C., Meyer, D., Loschonsky, S., Funk, LM., Neumann, P., Ficner, R., Brodhun, F., Müller, M. and Tittmann, K. (2015)Tuning and switching enantioselectivity of asymmetric carboligation in an enzyme through mutational analysis of a single hot spot. Chembiochem., Dec: 16(18)2580-4 [Abstract]PDB: [4ZP1]


      • Jung, S., Maritzen, T., Wichmann, C., Jing, Z., Neef, A., Revelo, NH., Al-Moyed, H., Meese, S., Wojcik, SM., Panou, I., Bulut, H., Schu, P., Ficner, R., Reisinger, E., Rizzoli, SO., Neef, J., Strenzke, N., Haucke, V. and Moser. T. (2015) Disruption of adaptor protein 2? (AP-2?) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing. EMBO J. , Nov 3; 34(21): 2686 - 702 [Abstract]

      • Dickmanns, A., Monecke, T. and Ficner, R. (2015) Structural Basis of Targeting the Exportin CRM1 in Cancer. Cells, 4(3), 538 - 568 Review. [Abstract]

      • Kuhle, B. Eulig, NK. and Ficner R. (2015) Architecture of the eIF2B regulatory subcomplex and its implications for the regulation of guanine nucleotide exchange on eIF2. Nucleic Acids Res. Nov 16; 43 (20): 9994 - 10014 [Abstract]; PDB:[4ZEM];[4ZEO];[5DBO]


      • Mahal, K., Ahmad, A., Sethi, S., Resch, M., Ficner, R., Sarkar, FH. , Schobert,R. and Biersack, B. (2015). Role of JNK and NF-?B in mediating the effect of combretastatin A-4 and brimamin on endothelial and carcinoma cells. Cell Oncol (Dordr).Dec; 38(6): 463 - 78. [Abstract]


      • Halder K., Dölker N., Van Q., Gregor I., Dickmanns A., Baade I., Kehlenbach RH., Ficner R., Enderlein J., Grubmüller H. and Neumann H.(2015).MD Simulations and FRET Reveal an Environment-Sensitive Conformational Plasticity of Importin-ß. Biophys 109(2):277-86.[Abstract]


      • Melin, J., Kilisch, M., Neumann, P., Lytovchenko, O., Gomkale, R.,Schendzielorz, A., Schmidt, B., Liepold, T., Ficner, R., Jahn, O., Rehling, P. and Schulz, C. (2015). A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria. Biochim Biophys Acta. May 7; 1853(8): 1850 - 1859 [Abstract]


      • Commichau,FM., Dickmanns, A., Gundlach, J., Ficner, R. and Stülke, J. (2015).A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.Mol Microbiol. 97(2):189-204. [Abstract]


      • Fischer, N., Neumann, P., Konevega, A.L., Bock, L.V., Ficner, R., Rodnina, M.V. and Stark, H. (2015). Structure of the E. coli ribosome-EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM. Nature, Apr 23;520(7548):567-70 [Abstract]


      • Rosenberg, J., Dickmanns,A., Neumann, P., Gunka, K., Arens, J., Kaever, V., Stülke, J., Ficner, R., and Commichau, F.M.(2015). Structural and biochemical analysis of the essential diadenylate cyclase CdaA from Listeria monocytogenes. J. Biol. Chem., Mar 6;290(10):6596-606. [Abstract]; PDB: [4RV7]


      • Gundlach, J., Dickmanns A., Schröder-Tittmann K., Neumann P., Kaesler J., Kampf J., Herzberg C., Hammer E., Schwede F., Kaever V., Tittmann K., Stülke J., and Ficner R. (2015). Identification, characterization and structure analysis of the c-di-AMP binding PII-like signal transduction protein DarA. J. Biol. Chem., Jan 30; 290 (5): 3069-80 [Abstract]; PDB: [4RLE]





      • 2014




        • Neumann, P. and Tittmann, K. (2014). Marvels of enzyme catalysis at true atomic resolution: distortions, bond elongations, hidden flips, protonation states and atom identities. Curr Opin Struct Biol. Dec;29:122-33. [Abstract]

        • Kuhle, B. and Ficner, R. (2014). Structural insight into the recognition of amino-acylated initiator tRNA by eIF5B in the 80S initiation complex. BMC Struct. Biol. Sep 17;14(1):20. [Abstract]

        • Kuhle, B. and Ficner, R. (2014). A monovalent cation acts as structural and catalytic cofactor in translational GTPases. EMBO J. Sep 15. pii: e201488517. [Abstract]; PDB: [4TMT]; [4TMV]; [4TMX]; [4TMW]; [4TMZ]; [4TN1]

        • Möhlmann, S., Mathew, R., Neumann, P., Schmitt, A., Lührmann, R. and Ficner, R. (2014). Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28. Acta Crystallogr. D70: 1622-1630. [Abstract] ; PDB: [4NHO]

        • Monecke, T., Dickmanns, A. and Ficner R. (2014) Allosteric control of the exportin CRM1 unraveled by crystal structure analysis. FEBS J., Sep;281(18):4179-94. [Abstract]

        • Liu Y, Neumann P, Kuhle B, Monecke T, Schell S, Chari A, Ficner R. (2014). Translation Initiation Factor eIF3b Contains a Nine-Bladed b-Propeller and Interacts with the 40S Ribosomal Subunit. Structure, 22:1-8. [Abstract]; PDB: [4NOX]

        • Neumann P, Lakomek K, Naumann PT, Erwin WM, Lauhon CT. and Ficner R. (2014). Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification. Nucleic Acids Res., 42:6673-6685. [Abstract] [Article] ; PDB: [4KR6] [4KR7] [4KR9]

        • Kuhle, B. and Ficner, R. (2014). eIF5B employs a novel domain release mechanism to catalyze ribosomal subunit joining. EMBO Journal, 10:1177-91 [Abstract]; PDB: [4NCF] [4NCL] [4NCN] [4NG3] [4N3N] [4N3S]

        • Mahal, K., Resch,M., Ficner, R., Schobert, R., Biersack, B. and Mueller, T. (2014). Effects of the Tumor-Vasculature-Disrupting Agent Verubulin and Two Heteroaryl Analogues on Cancer Cells, Endothelial Cells, and Blood Vessels. ChemMedChem, 4:847-54 [Abstract]

        • Ahmed, J.L. and Ficner, R. 2014. RNA synthesis and purification for structural studies. RNA Biol, 11:5 [Abstract]

        • Monecke T, Buschmann J, Neumann P, Wahle E. and Ficner R. (2014). Crystal Structures of the Novel Cytosolic 5'-Nucleotidase IIIB Explain Its Preference for m7GMP. PLoS One, 9:e90915. [Abstract]; [Article]; PDB: [4NV0] [4NWI]

        • Lehneck, R., Neumann, P., Vullo, D., Elleuche, S., Supuran, C.T., Ficner, R. and Pöggeler, S. (2014). Crystal structures of two tetrameric β-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora. FEBS J., 7:1759-72. [Abstract] ; PDB: [4O1K] [4O1J]

        • Khoshnevis, S.,L., Gunisová, S., Vlcková, V., Kouba, T., Neumann, P., Beznosková, P., Ficner, R. and Valasek, L.S. (2014). Structural integrity of the PCI domain of eIF3a/TIF32 is critically required for mRNA recruitment to the 43S pre-initiation complexes. Nucleic Acids Res., 42: 4123-4139. [Abstract];[Article]; PDB: [4K51]

        • Christian, H., Hofele, R., Urlaub, H. and Ficner , R. (2014). Insights into the activation of the helicase Prp43 by biochemical studies and structural mass spectrometry. Nucl. Acid Res., 42:1162-79 [Abstract]



        2013



        • Ahmed, Y.L., Gerke, J., Park, H.-S., Bayram, Ö., Neumann, P., Min Ni, M., Dickmanns, A., Kim, S.C. , Yu, J.-H., Braus, G.H. and Ficner, R. (2013). The Velvet Family of Fungal Regulators Contains a DNA-Binding Domain Structurally Similar to NF-kB. PlosBiol., 11(12): e1001750 [Abstract] [Article] ; PDB: [4N6Q] [4N6R].

          Roberts RG (2013). The Velvet Underground Emerges. PLoS Biol 11(12): e1001751. [Synopsis to Ahmed et al. in PloS Biol]

        • Xiang, S.Q. , Gapsys, V., Kim, H.-Y., Bessonov, S., Hsiao,H.-H., Möhlmann, S., Klaukien, K., Ficner, R., Becker, S., Urlaub, H., Lührmann, R., de Groot, B. and Zweckstetter, M. (2013). Phosphorylation drives a dynamic switch in serine/arginine-rich proteins. Structure, 12:2162-74 [Abstract]

        • Lüdtke, S., Neumann, P., Erixon, K.M., Kluger, R., Ficner, R. and Tittmann, K. (2013). Sub-ångström-resolution crystallography reveals physical distortions that enhance reactivity of a covalent enzymatic intermediate. Nat. Chem., 5:762-767 [Abstract]; PDB: [4KXV] [4KXW] [4KXU] [4KXX] [4KXY]

        • Dölker, N., Blanchet, C.E., Voß, B., Haselbach, D., Kappel , C., Monecke, T., Svergun, D.I., Stark, H., Ficner, R., Zachariae, U., Grubmüller, H. and Dickmanns, A. (2013) . Structural determinants and mechanism of mammalian CRM1 allostery. Structure, 21:1350-1360. [Abstract]; EMDB: [EMD-2274] [EMD-5564]

        • Meyer, D., Neumann, P., Ficner, R. and Tittmann, K. (2013). Observation of a stable carbene at the active site of an enzyme. Nat. Chem. Biol., 9:488-490.[Abstract]; PDB: [4KGD]

        • Naumann, P.-T., Lauhon, C.T. and Ficner, R. (2013). Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase - RNA complex. Acta Cryst. F69: 421-424. [Abstract]

        • Volkov, A., Khoshnevis, S., Neumann, P., Herrfurth, C., Wohlwend, D., Ficner, R. and Feussner, I. (2013). Crystal structure analysis of a fatty acid double bond hydratase from Lactobacillus acidophilus. Acta Crystallogr. D69: 648-657 [Abstract]; [4IA5][4IA6]

        • Fourmann, J.B., Schmitzová, J., Christian, H., Urlaub, H., Ficner, R., Boon, K.-L., Fabrizio, P., and Lührmann, R. (2013). Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing system. Genes&Dev. 27: 413-428. [Abstract]

        • Resch, M., Göpel, Y., Görke, B. and Ficner R. (2013). Crystallization and preliminary X-ray diffraction analysis of YhbJ from Escherichia coli, a key protein involved in the GlmYZ sRNA regulatory cascade. Acta Cryst. F69: 109-114. [Abstract]

        • Monecke, T., Haselbach, D., Voß, B., Russek, A., Neumann, P., Thomson, E., Hurt, E., Zachariae, U., Stark, H., Grubmüller, H., Dickmanns, A. and Ficner, R. (2013). Structural basis for cooperativity of CRM1 export complex formation. PNAS USA. 110: 960-965. [Abstract]; [Publication]; PDB: [4FGV], [4HZK], EMDB: [EMD-2110], [EMD-2111]



        2012



        • Khoshnevis, S., Hauer, F., Milón, P., Stark, H., and Ficner, R. (2012). Novel insights into the architecture and protein interaction network of yeast eIF3. RNA 18: 2036-2319.[Abstract]

        • Neumann, P., Brodhun, F., Sauer, K., Herrfurth, C., Hamberg, M., Brinkmann, J., Scholz, J., Dickmanns, A., Feussner, I. and Ficner R. (2012). Crystal structures of Physcomitrella patens AOC1 and 2: Insights into the enzyme mechanism and differences in substrate specificity. Plant Physiol. 160: 251-1266. [Abstract]; PDB: [4H69], [4H6A], [4H6B], [4H6C]

        • Kudlinzki, D., Schmitt, A., Christian, H. and Ficner, R. (2012) Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22. Biol. Chem. 393: 1131?1140. [Abstract]; PDB: [3I4U]

        • Bayram, Ö., Ahmed, Y.L., Ficner, R. and Braus G.H. (2012). The A. nidulans MAPK module AnSte11-Ste50-Ste7-Fus3 controls development and secondary metabolism. PLoS Genetics. 8(7): e1002816. [Abstract]; [Publication]

        • Meyer D., Neumann P., Koers E., Sjuts H., Lüdtke S., Sheldrick G.M., Ficner R., Tittmann K. (2012). Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin. PNAS USA.109: 10867-72. [Abstract]; PDB: [4FEG] , [4FEE]

        • Schulz, E.-C., Roth H.M., Ankri, S. and Ficner, R. (2012).
          Structure Analysis of Entamoeba histolytica DNMT2 (EhMeth). PlosONE.7(6):e38728 [Abstract]; [Publication]; PDB: [3QV2]



        2011



        • Schulz C., Lytovchenko O., Melin J., Chacinska A., Guiard B., Neumann P., Ficner R., Jahn O., Schmidt B., Rehling P. (2011). Tim50's presequence receptor domain is essential for signal driven transport across the TIM23 complex.
          J Cell Biol. 195(4):643-56. [Abstract]

        • Lehwess-Litzmann A., Neumann P., Parthier C., Lüdtke S., Golbik R., Ficner R. & Tittmann K. (2011). Twisted Schiff base intermediates and substrate locale revise transaldolase mechanism. Nat Chem Biol. 7(10):678-84. [Abstract]; PDB: [3S0C][3S1U][3S1V][3S1W][3S1X]

        • Schulz E.-C., Tietzel M., Tovy A., Ankrib S., Ficner R. (2011). Structure analysis of Entamoeba histolytica enolase. Acta Cryst. D67: 619?-627. [Abstract]; PDB: [3QTP]

        • Schulz E.-C., Bergfeld, A.K., Ficner R. and Mühlenhoff M. (2011). Structural basis for capsule form-variation in Escherichia coli K1: the crystal structure of polySia specific O-acetyltransferase NeuO. PLoS One. 6(3):e17403. [Abstract]; PDB: [3JQY]

        • Schulz E.-C. and Ficner R. (2011). Knitting and snipping: chaperones in β-helix folding. Curr Opin Struct Biol. (2):232-9. [Abstract]

        • Helfmann S., Neumann P., Tittmann K., Moser T., Ficner R., Reisinger E. (2011). The Crystal Structure of the C(2)A Domain of Otoferlin Reveals an Unconventional Top Loop Region. J Mol Biol. 406(3):479-90. [Abstract]; PDB: [3L9B]

        • Dickmanns A., Damerow S., Neumann P.; Schulz E.-C., Lamerz A.-C., Routier H. F., Ficner R. (2011). Structural basis for the broad substrate range of the UDP-sugar pyrophosphorylase from Leishmania major. J.Mol.Biol. 405(2):461-78. [Abstract]; PDB: [3OGZ][3OH0][3OH1][3OH2][3OH3][3OH4]



        2010



        • Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M. & Görlich D. (2010). NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Nature Struct. Mol. Biol. (11):1367-76. [Abstract]; PDB: [3NBY][3NBZ][3NC0][3NC1]

        • Khoshnevis S., Neumann P., Ficner, R. (2010). Crystal Structure of the RNA Recognition Motif of Yeast Translation Initiation Factor eIF3b Reveals Differences to Human eIF3b. PLoS ONE 5(9): e12784. [Abstract]; PDB: [3NS6][3NS5]

        • Lakomek, K., Dickmanns, A., Ciirdaeva, E., Schomacher, L. and Ficner, R. (2010). Crystal structure analysis of DNA uridine endonuclease Mth212 bound to DNA. J Mol Biol. 18;399(4):604-17. [Abstract]; PDB: [3G00][3G0A][3G0R][3G1K][3G2C][3G2D][3G38][3G3C][3G3Y][3G4T][3G8V][3G91][3FZI][3GA6]

        • Kühn-Hölsken, E., Lenz, C., Dickmanns, A., Hsiao, H.-H., Richter, F.M., Kastner, B., Ficner, R., and Urlaub, H. (2010). Mapping the binding site of Snurportin 1 on native U1 snRNP by cross-linking and mass spectrometry. Nucleic Acids Res. 38(16):5581-93. [Abstract]

        • Schulz, E.-C., Schwarzer, D, Frank, M., Stummeyer, K., Mühlenhoff, M., Dickmanns, A., Gerardy-Schahn, R., and Ficner, R. (2010). Structural basis for recognition and cleavage of polysialic acid by the bacteriophage K1F tailspike protein endoNF. J. Mol. Biol., 937: 341-51. [Abstract]; PDB: [3GVJ][3GVK][3GVL]

        • Schulz, E.-C., Dickmanns, A., Urlaub, H., Schmitt, A., Mühlenhoff, M., Stummeyer, K., Schwarzer, D., Gerardy-Schahn, R., and Ficner, R. (2010). Crystal structure of a novel intramolecular chaperone mediating triple ß-helix folding. Nature Struct. Mol. Biol., 17:210-5. [Abstract]; PDB: [3GUD][3GW6]

        • Schulz, E.-C., Neumann, P., Gerardy-Schahn, R., Sheldrick, G.M., and Ficner, R. (2010). Crystal structure analysis of endosialidaseNF at 0.98 Å resolution. Acta Crystallogr. D66: 176-80. [Abstract]; PDB: [3JU4]

        • Khoshnevis, S., Gross, T., Rotte, C., Baierlein, C., Ficner, R., and Krebber, H. (2010). The iron-sulfur protein Rli1 functions in translation termination. EMBO Rep., 11: 214- 9 [Abstract]



        2009




        • Warkocki, Z., Odenwälder, C., Schmitzova, J., Platzmann, F., Stark, H., Urlaub, H., Ficner, R, Fabrizio, P. and Lührmann, R. (2009). Reconstitution of both Steps of Saccharomyces cereviseae splicing with purified spliceosomal components. Nature Struct. Mol. Biol. 12: 1237-43. [Abstract]

        • Kudlinzki, D., Nagel, C., and Ficner, R. (2009). Crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of the human spliceosomal DExD/H-box protein hPrp22.Acta Crystallogr. F 65: 956-958. [Abstract]

        • Lakomek, K., Dickmanns, A., Kettwig, M., Urlaub, H., Ficner, R., and Luebke, T. (2009). Initial insight into the function of the lysosomal 66.3 kDa protein from mouse by means of X-ray crystallography. BMC Struct Biol.,9: 56. [Abstract]; PDB: [3FGR][3FGT][3FGW]

        • Oschlies, M., Dickmanns, A., Haselhorst, T., Schaper, W., Stummeyer, K., Tiralongo, J., Weinhold, B., Gerardy-Schahn, R., von Itzstein, M., Ficner, R., and Münster-Kühnel, A.K. (2009). A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for the physiologically active enzyme. J. Mol. Biol. 393:83-97. [Abstract]; PDB: [3EWI]

        • Goette, M., Stumpe, M.C., Ficner, R. and Grubmüller, H. (2009). Molecular determinants of snurportin1 ligand affinity and structural response upon binding. Biophys. J. 97: 581-589. [Abstract] PDB: [1XK5]

        • Monecke, T., Dickmanns, A., and Ficner, R. (2009). Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1. Nucleic Acids Res. 37: 3865-3877. [Abstract]; PDB: [3GDH]

        • Ficner, R. (2009). Novel structural insights into class I and II histone deacetylases. Curr. Top. Med. Chem. 9: 235-240. [Abstract]

        • Monecke, T., Güttler, T., Neumann, P., Dickmanns, A., Görlich, D., and Ficner, R. (2009). Crystal structure of the nuclear export receptor CRM1 in complex with snurportin1 and RanGTP. Science, 324:1087-1091. [Abstract]; PDB: [3GJX]

        • Monecke,T., Dickmanns, A., Strasser A., and Ficner R. (2009). Structure Analysis of the conserved Methyltransferase Domain of Human Trimethylguanosine Synthase TGS1. Acta Crystallogr. D65: 332-338. [Abstract]; PDB: [3EGI]

        • Lakomek, K., Dickmanns, A., Müller, U., Kollmann, K., Deuschl, F., Berndt, A., Lübke, T., and Ficner, R. (2009). De novo sulfur SAD phasing of the lysosomal 66.3 kDa protein from mouse. Acta Crystallogr. D 65: 220-8. [Abstract]; PDB: [3FBXI]

        • Padmanabhan, N., Fichtner, L., Dickmanns, A., Ficner, R., Schulz, J.B., and Braus, G. (2009). The Yeast HtrA Orthologue Ynm3 Is a Protease with Chaperone Activity that Aids Survival under Heat Stress.Mol.Cell. Biol., 20: 68-77. [Abstract]



        2008




        • Monecke,T., Schell, S., Dickmanns, A., and Ficner R. (2008). Crystal structure of the RRM domain of poly(A)-specific ribonuclease reveals a novel m7G-cap binding mode. J. Mol. Biol. 382: 827-34. [Abstract]; PDB: [3CTR]

        • Mathew, R., Hartmuth, K., Möhlmann, S., Urlaub, H., Ficner, R., and Lührmann, R. (2008). Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome. Nature Struct. Mol. Biol. 15: 435-443. [Abstract]

        • Schäfer, S., Saunders, L., Eliseeva, E., Velena, A., Jung, M., Schwienhorst, A., Strasser, A., Dickmanns, A., Ficner, R., Schlimme, S., Sippl, W., Verdin, E., and Jung, M. (2008). Phenylalanine-containing hydroxamic acids as selective inhibitors of class IIb histone deacetylases (HDACs). Bioorg. Med. Chem. 16: 2011-2033. [Abstract]; PDB: [2VGC]



        2007




        • Wohlwend, D., Strasser, A., Dickmanns, A., and Ficner R. (2007). Structural Basis for RanGTP Independent Entry of Spliceosomal U snRNPs into the Nucleus. J. Mol. Biol. 374: 1129-1138. [Abstract]; PDB: [2QNA]

        • Nielsen, T.K., Liu, S., Lührmann, R. and Ficner, R. (2007). Structural basis for the bifunctionality of the U5 snRNP 52K protein (CD2BP2). J. Mol. Biol. 369: 902-908. [Abstract]; PDB: [1SYX]

        • Nielsen, T.K., Hildmann, C., Riester, D., Wegener, D., Schwienhorst, A. and Ficner, R. (2007). Complex structure of a bacterial class 2 histone deacetylase homologue with a trifluoromethylketone inhibitor. Acta Crystallogr. F63: 270-273. [Abstract]; PDB: [2GH6]

        • Wohlwend, D., Strasser, A., Dickmanns, A., Doenecke, D. and Ficner R. (2007). Thermodynamic analysis of H1 nuclear import: receptor tuning of importinbeta/importin7. J. Biol. Chem. 282: 10707-10719. [Abstract]



        2006




        • Grimm, C., Ficner, R., Sgraja, T., Haebel, P., Klebe, G. and Reuter, K. (2006). Crystal structure of Bacillus subtilis S-adenosylmethionine:tRNA ribosyl-transferase- isomerase. Biochem. Biophys. Res. Commun. 351: 695-701. [Abstract]; PDB: [1YY3I]

        • Hildmann, C., Wegener, D., Riester, D., Hempel, R., Schober, A., Merana, J., Giurato, L., Guccione, S., Nielsen, T.K., Ficner, R. and Schwienhorst, A. (2006). Substrate and inhibitor specificity of class 1 and class 2 histone deacetylases.
          J. Biotechnol. 124: 258-70. [Abstract]

        • Dickmanns, A., Ballschmitter, M., Liebl, W. and Ficner, R. (2006). Crystal structure of the novel α-amylase AmyC from Thermotoga maritima. Acta Crystallogr. D62: 262-270. [Abstract]; PDB: [2B5D]



        2005




        • Nielsen, T.K., Hildmann, C., Dickmanns, A., Schwienhorst, A. and Ficner, R. (2005). Crystal structure of a bacterial class 2 histone deacetylase.J. Mol. Biol. 354: 107-120. [Abstract]; PDB: [1ZZ0][1ZZ1][1ZZ3]

        • Andrade, S. L. A., Dickmanns, A., Ficner, R. and Einsle, O. (2005). Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus.PNAS. USA, 102: 14994-14999. [Abstract]; PDB: [2B2F][2B2H][2B2I][2B2J]

        • Andrade, S. L. A., Dickmanns, A., Ficner, R. and Einsle, O. (2005). Expression, Purification and Crystallization of the Ammonium Transporter Amt-1 from Archaeoglobus fulgidus. Acta Crystallogr. F61: 861-863. [Abstract]

        • Strasser, A., Dickmanns, A., Lührmann, R. and Ficner, R. (2005). Structural basis for m3G-cap mediated nuclear import of spliceosomal UsnRNPs by Snurportin 1. EMBO J. 24: 2235-2243. [Abstract]; PDB: [1XK5]

        • Dierks, T., Dickmanns, A., Preusser-Kunze, A., Schmidt, B., Mariappan, M., von Figura, K., Ficner, R. and Rudolph, M.G. (2005). Molecular basis for multiple sulfatase deficiency and catalytic mechanism for formylglycine generation of the human formylglycine generating enzyme. Cell 121: 541-552. [Abstract]; PDB: [1Y1E][1Y1F][1Y1G][1Y1H][1Y1I][1Y1J]

        • Dickmanns, A., Schmidt, B., Rudolph, M.G., Mariappan, M., Dierks, T., von Figura, K. and Ficner, R. (2005). Crystal structure of human pFGE, the paralogue of the Ca-formylglycine generating enzyme. J. Biol. Chem. 280: 15180-15187. [Abstract]; PDB: [1Y4J]

        • Dickmanns, A. and Ficner, R. (2005). Role of the 5?-cap in the biogenesis of spliceosomal snRNPs. Topics in Current Genetics 12: 179-204.


        • Arnorsdottir, J., Kristjanson, M.M. and Ficner, R. (2005). Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation. FEBS J. 272: 832-845. [Abstract]; PDB: [1S2N][1S7H]

        • Stummeyer, K., Dickmanns, A., Mühlenhoff, M., Gerardy-Schahn, R. and Ficner, R. (2005). Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F. Nature Struct. Mol. Biol. 12: 90-96. [Abstract]; PDB: [1V0E][1V0F]



        2004




        • Strasser, A., Dickmanns, A., Schmidt, U., Penka, E., Urlaub, H., Sekine, R., Lührmann, R. and Ficner, R. (2004). Purification, crystallization and preliminary crystallographic data of the m3G cap-binding domain of human snRNP import factor Snurportin1. Acta Crystallogr. D60: 1628-1631. [Abstract]; PDB: [1XK5]



        2003




        • Rudolph, M.G., Kraus, I., Dickmanns, A., Eickmann, M., Garten, W. and Ficner, R. (2003). Crystal Structure of the Borna Disease Virus Nucleoprotein. Structure 11: 1219-1226. [Abstract]; PDB: [1N93][1PP1]

        • Ingelfinger, D., Gothel, S.F., Marahiel, M.A., Reidt, U., Ficner, R., Lührmann, R. and Achsel, T. (2003).Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH. Nucleic Acids Res. 31: 4791-4796. [Abstract]

        • Reidt, U., Wahl, M.C., Fasshauer, D., Horowitz, D.S., Lührmann, R. and Ficner, R. (2003).Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide. J. Mol Biol. 331: 45-56. [Abstract]; PDB: [1MZW]



        2001




        • Grädler, U., Gerber, H.-D., Goodenough-Lashua, D.M., Garcia, G.A., Ficner, R., Reuter, K., Stubbs, M.T. and Klebe, G. (2001). A new target for shigellosis: rational design and crystallographic studies of inhibitors of tRNA-guanine transglycosylase. J. Mol. Biol. 306: 455-467. [Abstract]; PDB: [1F3E][1ENU]

        • Maser, E., Xiong, G., Grimm, C., Ficner, R. and Reuter, K. (2001). 3α-Hydroxy­steroid dehydrogenase/ carbonyl reductase from Comomonas testosteroni: biological significance, three-dimensional structure and gene regulation. Chem.-Biol. Interact. 130-132(1-3):707-22. [Abstract]



        2000




        • Vidovic, I., Nottrott, S., Hartmuth, K., Lührmann, R. and Ficner, R. (2000). Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment. Mol. Cell 6: 1331-1342. [Abstract]; PDB: [1E7K]

        • Grimm, C., Maser, E., Möbus, E., Klebe, G., Reuter, K. and Ficner, R. (2000). The crystal structure of 3α-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni shows a novel oligomerisation pattern within the short chain dehydrogenase/ reductase family. J. Biol. Chem. 275: 41333-41339. [Abstract]; PDB: [1FJH][1FK8]

        • Grimm, C., Klebe, G., Ficner, R. and Reuter, K. (2000). Screening orthologs as an important variable in crystallization: Preliminary X-ray diffraction studies on the tRNA-modifying enzyme S-adenosylmethionine:tRNA-transferase/isomerase. Acta Crystallogr. D56: 484-488. [Abstract]

        • Reidt, U., Reuter, K., Ingelfinger D., Achsel, T., Lührmann, R. and Ficner, R. (2000). Crystal structure of the human U4/U6 small nuclear ribonucleoprotein particle-specific SnuCyp-20, a nuclear cyclophilin. J. Biol. Chem. 275: 7439-7442. [Abstract]; PDB: [1QOI]



        1999




        • Reuter, K., Mofid, M.R., Marahiel, M.A. and Ficner, R. (1999). Crystal structure of the surfactin synthetase activating enzyme Sfp: a prototype of the 4'-phospho- pantetheinyl transferase superfamily. EMBO J. 18: 6823-6831. [Abstract]; PDB: [1QR0]

        • Reuter, K., Nottrott, S., Fabrizio, P., Lührmann, R. and Ficner, R. (1999). Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein. J. Mol. Biol. 294: 515-525. [Abstract]; PDB: [1QGV]

        • Nottrott, S., Hartmuth, K., Fabrizio, P., Urlaub, H., Vidovic, I., Ficner, R. and Lührmann, R. (1999). Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5?stem-loop of U4 snRNA. EMBO J. 18: 6119-6133. [Abstract]; PDB: [1E7K]

        • Mofid, M.R., Marahiel, M., Ficner, R. and Reuter, K. (1999). Crystallization and preliminary crystallographic studies of Sfp: a phosphopantetheinyl transferase of modular peptide synthetases. Acta Crystallogr. D55: 1098-1100. [Abstract]; PDB: [1QR0]

        • Reuter, K. and Ficner, R. (1999). Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human spliceosomal protein U5-15kD. Acta Crystallogr. D55: 888-890. [Abstract]; PDB: [1QGV]

        • Grädler, U., Ficner, R., Garcia, G.A., Stubbs, M.T., Klebe, G. and Reuter, K. (1999). Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity. FEBS Lett. 454: 142-146. [Abstract]; PDB: [1EFZ]