Kramer, Wilfried, PD Dr.
Privatdozent Molecular Biology and Genetics
- Diploma (Biology), University of Cologne, Germany, 1982
- Dr. rer. nat., University of Cologne, Germany, 1986
- Postdoctoral Fellow, University of California, Berkeley, USA, 1986 - 1989
- Habilitation in Molecular Biology and Genetics, University of Göttingen, Germany, 2000
- At the Dept. of Molecular Genetics since 1989
Major Research Interests
In the Department of Molecular Genetics, headed by Prof. Dr. H. Krebber, I try to identify new factors that might be involved in the export of mRNA from the nucleus in Saccharomyces cerevisiae. To this end, ordered mutants arrays are screened for genetic interactions with selected mutants by the so called SGA technique, which makes use of the genetic features offered by budding yeast to rapidly construct double mutants and compare their growth with that of single mutants. Furthermore, we want to extend these studies in different collaborations to microscopic screenings of those mutant arrays for export defects using automated microscopes. In a collaboration with Prof. Dr. S. Emmert from the medical faculty we want to analyse the function of the yeast MPH1 gene and of its human homologue FANCM. The latter is a determining factor of the hereditary disease Fanconi anemia, which is – besides other symptoms - characterised by chromosome instability and increased incidence of cancer. Both are associated to homologous recombination and at least Mph1 is very likely involved in the error-free bypass of lesions, which are caused by DNA damaging agents and are blocking DNA replication, posing a very serious threat to the survival of the cell. Understanding these cellular responses to DNA damage will allow a better insight into central processes involved in the malignant transformation of cells.
Homepage Department/Research Group
Selected Recent Publications
- Ede C, Rudolph CJ, Lehmann S, Schürer KA, Kramer W (2011) Budding yeast Mph1 promotes sister chromatid interactions by a mechanism involving strand invasion. DNA Repair 10, 45-55.
- Schomacher L, Schürer KA, Ciirdaeva E, McDermott P, Chong J, Kramer W, Fritz HJ (2010) Archaeal DNA uracil repair via direct strand incision: A minimal system reconstituted from purified components. DNA Repair 9, 438-447.
- Panico ER, Ede C, Schildmann M, Schürer KA, Kramer W (2010) Genetic evidence for a role of Saccharomyces cerevisiae Mph1 in recombinational repair under replicative stress. Yeast 27, 11-27.
- Prakash R, Satory D, Dray E, Papusha A, Scheller J, Kramer W, Krejci L, Klein H, Haber JE, Sung P, Ira G (2009) Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Genes Dev 23, 67-79.
- Schürer KA, Rudolph C, Ulrich HD, Kramer W (2004) Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from homologous recombination, but not from postreplication repair. Genetics 166, 1673-1686.