Jakobs, Stefan, Prof. Dr.
Professor of High Resolution Microscopy in Neurodegenerative Diseases
- 1995 - Diploma, University of Kaiserslautern
- 1995 - 1999 Graduate studies (MPI for Plant Breeding Research, Cologne, Germany and John-Innes-Centre, Norwich, GB)
- 1999 Dr. rer. nat. University of Cologne
- 1999 Postdoc (Laboratory of J. Schell/K. Palme, MPI for Plant Breeding Research, Cologne)
- 1999 - 2005 Postdoc (MPI for Biophysical Chemistry, Laboratory of S.W. Hell)
- 2005 - Research group leader at the MPI for Biophysical Chemistry
- 2007 - Habilitation (Botany/Cell Biology) at the Georg-August-University Göttingen
- 2010 - Professor (W2) of High Resolution Microscopy in Neurodegenerative Diseases,
University of Göttingen Medical School, Dept. of Neurology
Major Research Interests
Our two major research interests are the investigation of the nanoscale architecture and dynamics of mitochondria and the analysis of reversibly switchable fluorescent proteins (RSFPs) as probes for super-resolution microscopy. Mitochondria are essential organelles in all eukaryotic cells and their dysfunction is involved in many devastating (neurodegenerative) diseases. We want to understand the organization of mitochondria on the nanoscale in healthy and challenged cells and investigate the molecular mechanisms that determine their intricate structure. We utilize a wide array of techniques, including molecular biology, biochemical methods as well as electron and super-resolution microscopy.
RSFPs are fluorescent proteins that may be switched by light between a non-fluorescent and a fluorescent state. Their unique properties open up numerous applications in microscopy and cell biology. We investigate the molecular switching mechanisms and aim to improve the properties of these fascinating proteins as probes for live-cell super-resolution microscopy
Homepage Department/Research Group
Selected Recent Publications
- Sahl SJ, Hell SW, Jakobs S (2017) Fluorescence nanoscopy in cell biology, Nature Rev Mol Cell Biol doi:10.1038/nrm.2017.71.
- Große L, Wurm CA, Brüser C, Neumann D, Jans DC, Jakobs S (2016) Bax assembles into large ring-like structures remodeling the mitochondrial outer membrane in apoptosis. EMBO J 35: 402-413.
- Sahl SJ, Balzarotti F, Keller-Findeisen J, Leutenegger M, Westphal V, Egner A, Lavoie-Cardinal F, Chmyrov A, Grotjohann T, Jakobs S (2016) Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics". Science 352: pp. 527.
- Schnorrenberg S, Grotjohann T, Vorbrüggen G, Herzig A, Hell SW, Jakobs S (2016) In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster. Elife 5: e15567.
- Jans DC, Wurm CA, Riedel D, Wenzel D, Stagge F, Deckers M, Rehling P, Jakobs S (2013) STED super-resolution microscopy reveals an array of MINOS clusters along human mitochondria. Proc Natl Acad Sci U S A 110: 8936-8941.
- Grotjohann T, Testa I, Reuss M, Brakemann T, Eggeling C, Hell SW, Jakobs S (2012) rsEGFP2 enables fast RESOLFT nanoscopy of living cells. Elife 1: e00248.
- Brakemann T, Stiel AC, Weber G, Andresen M, Testa I, Grotjohann T, Leutenegger M, Plessmann U, Urlaub H, Eggeling C, Wahl MC, Hell SW, Jakobs S (2011) A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Nature Biotech 29: 942-947 .
- Grotjohann T, Testa I, Leutenegger M, Bock H, Urban NT, Lavoie-Cardinal F, Willig KI, Eggeling C, Jakobs S*, Hell SW* (2011) (*shared corr.) Diffraction-unlimited all-optical imaging and writing with a photochromic GFP. Nature 478: 204-208.
- Wurm CA, Neumann D, Lauterbach MA, Harke B, Egner A, Hell SW, Jakobs S (2011) Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient. Proc Natl Acad Sci U S A 108: 13546-13551.
- Andresen M, Stiel AC, Folling J, Wenzel D, Schönle A, Egner A, Eggeling C, Hell SW, Jakobs S (2008) Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy. Nature Biotechnol 26: 1035-1040.