Veranstaltung
A curious case of co-evolution: The story of RNA processing in human mitochondria| Titel der Veranstaltung | A curious case of co-evolution: The story of RNA processing in human mitochondria |
| Reihe | campus seminar |
| Veranstalter | Max-Planck-Institut für biophysikalische Chemie (MPIBPC) |
| Referent/in | Arjun Bhatta |
| Einrichtung Referent/in | Research Group Structure and Function of Molecular Machines |
| Veranstaltungsart | Seminar |
| Kategorie | Forschung |
| Anmeldung erforderlich | Nein |
| Beschreibung | The bulk of ATP required to power our cells is produced by the oxidative phosphorylation (OXPHOS) system in our mitochondria. Assembly of this OXPHOS apparatus requires concerted expression of both nuclear and mitochondrial genome. RNAs transcribed from our mitochondrial genomes are polycistronic, and contain messenger and ribosomal RNAs punctuated by transfer RNAs (tRNAs). These tRNA within the polycistronic transcripts are excised by mitochondrial RNase (mtRNase) P and Z to liberate all RNA species. Unlike nuclear or bacterial RNase P, mtRNase P is not a ribozyme but instead consists of three protein subunits that together carry out RNA recognition, cleavage and methylation by unknown mechanisms. Here we present the cryo-electron microscopy structure of human mtRNase P bound to precursor tRNA, which reveals a unique mechanism of substrate recognition and processing. The subunits TRMT10C and SDR5C1 form a subcomplex that binds conserved elements in mitochondrial tRNAs, including the anticodon loop, and positions the tRNA for methylation by TRMT10C. The endonucleolytic subunit PRORP is recruited and activated through interactions with TRMT10C, which ensures precise pre-tRNA cleavage. The structure elucidates the mechanistic basis of mitochondrial RNA processing and suggests coevolution of tRNAs and RNase P in human mitochondria. |
| Zeit | Beginn: 02.02.2022, 11:00 Uhr Ende: 02.02.2022 , 12:00 Uhr |
| Ort |
Max-Planck-Institut für biophysikalische Chemie (MPIBPC) (Am Faßberg 11) Online |
| Kontakt |
Stefan Glöggler stefan.gloeggler@mpibpc.mpg.de |