Eduardo Cienfuegos Pecina

EDUCATION

College / University

Universidad Autónoma de Nuevo León

Highest Degree

Bachelor of Science

Major Subjects

Clinical Chemistry, Biochemistry, Hepatology, Organic Chemistry, Molecular Biology

CienfuegosPecina

Country

Mexico

Lab Experience

In vivo surgical models of liver and kidney ischemia-reperfusion injury in rats. Immunological techniques: ELISA, Western blot. Molecular biology: total protein, DNA and RNA extraction, purification, and quantification; gel electrophoresis, PCR, RT-qPCR. Organic chemistry: Organic synthesis, column and thin-layer chromatography. Spectroscopic analysis: Structural elucidation by 1H and 13C nuclear magnetic resonance, 1H-NMR-Based metabolomics, polarimetry. Clinical laboratory routine techniques in hematology, clinical chemistry, microbiology, urinalysis, and transfusion medicine. Basics of mammalian cell culture: passaging adherent cultures, cryopreservation, viability, and cytotoxicity assays

Projects / Research

  • 2018 – 2020: "Effect of inhibitors of the EGLN family of prolyl-4-hydroxylases against hepatic and renal ischemia-reperfusion injury in Wistar rats, involving a 1H-NMR-based metabolomics approach". University Hospital “Dr. José E. González”, Universidad Autónoma de Nuevo León
  • 2020: Effect of sodium (S)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway. PeerJ. 2020;8:e9438
  • 2018: Nephroprotective effect of Sonchus oleraceus extract against kidney injury induced by ischemia-reperfusion in Wistar rats. Oxidative Medicine and Cellular Longevity. 2018; ID 9572803

Scholarships / Awards

2020 – 2021: Stipend by the International Max Planck Research School.
2019: Academic Merit Award by the Universidad Autónoma de Nuevo León
2019: Award to the Excellent Performance in the National General Degree Examination
2018: Academic exchange at the University of Buenos Aires, Argentina

SCIENTIFIC INTERESTS AND GOALS

My main interest is to understand the role of oxygen metabolism in the physiopathology of ischemia-reperfusion injury, chronic liver disease, and cancer. Alterations in oxygen metabolism induced by the up or downregulation of the Hypoxia-Inducible Factors have been demonstrated to either drive the course of these pathologies or be a therapeutic strategy, as they are highly involved with the action of proto-oncogenes and tumor suppressor genes. Untargeted analytical techniques, such as NMR-based metabolomics, allow us to characterize the effect of treatments regulating the oxygen metabolism pathways and to identify secondary pathways affected by the disease or the treatment itself.