Monitoring changes in cytokinin localization pattern in relation to plant growth during seasons and drought stress using transgenic poplar reporter lines
Duration of the project: 2012 - 2016
Contact: Prof. Dr. Andrea Polle, Email: apolle(at)gwdg.de
Phytohormones play an important role in wood formation. Cytokinins are of particular interest, as they are considered to be the 'master regulators' of plant growth. The research goal of this Ph.D. project is to analyze how cytokinins bring about changes in wood formation and biomass allocation among tissues, in relation to its responsiveness and localization pattern, under various treatments, especially under drought stress. Transgenic poplar reporter lines are used for these studies. The main aim is to establish these reporter lines as an excellent system to investigate the role of cytokinins in tree growth and development which will enhance our current knowledge on plant growth adaptation to environmental factors. This can ultimately lead to a higher biomass production. The study is conducted as a part of WATBIO (Development of improved perennial non-food biomass and bioproduct crops for water stressed environments, www.watbio.eu) which is a collaborative research project.
Posters
Paul S, Wildhagen H, Polle A (2015) ARR5::GUS activity in Populus reporter lines in relation to growth and drought. Third WATBIO annual meeting, Crete, Greece (21-24 Sep 2015)
Wildhagen H, Smith HK, Allwright M, Viger M, Valdes-Fragoso M, Douthe C, Cohen D, Brendel O, Le Thiec D, Hummel I, Bure C, Janz D, Paul S, Haworth M, Pollastri S, Della Rocca G, Rüger S, Paulo J, Schnabel S, Scalabrin S, Vendramin V, Cattonaro F, Malinowska M, Robson P, Centritto M, Loreto F, Keurentjes J, van Eeuwijk F, Morgante M, Flexas J, Bogeat-Triboulot M-B, Taylor G, Polle A (2015) Investigating the molecular basis of drought tolerance in Populus: The WATBIO Populus core experiment. Third WATBIO annual meeting, Crete, Greece (21-24 Sep 2015)
Yu D, Paul S, Wildhagen H, Janz D, Bogeat-Triboulot M-B, Taylor G, Polle A (2015) Hormonal and molecular basis of growth and wood formation in Populus under drought stress. Conference on 'Perennial biomass crops for a resource constrained world', Stuttgart-Hohenheim, Germany (7-10 Sep 2015)
Paul S, Janz D, Wildhagen H, Teichmann T, Hänsch R, Polle A (2013) Cytokinin localization patterns in relation to growth and drought in Populus reporter lines. PRO-BIOPA Conference, Freising, Germany (27-29 Nov 2013)
Wildhagen H, Paul S, Janz D, Teichmann T, Hänsch R, Bogeat-Triboulot M-B, Taylor G, Polle A (2013) Effects of drought stress on growth and wood formation in Populus. First WATBIO annual meeting, Florence, Italy (30 Sep - 02 Oct 2013)
Presentations
Paul S, Wildhagen H, Polle A (2015) Tissue and cell-specific cytokinin activity monitored by an ARR5::GUS reporter construct in poplar wood. Molecular Plant Sciences Seminar, Schwann-Schleiden-Forschungszentrum, University of Göttingen, Germany (3 Dec 2015)
Paul S, Wildhagen H, Polle A (2015) Tissue and cell-specific cytokinin activity in poplar monitored by ARR5::GUS reporter lines. Forstbotanisches Seminar, Department of Forest Botany and Tree Physiology, University of Göttingen, Germany (23 Nov 2015)
Paul S, Wildhagen H, Polle A (2015) Seasonal fluctuation of cytokinin activity in poplar reporter lines under ambient conditions. Forstbotanisches Seminar, Department of Forest Botany and Tree Physiology, University of Göttingen, Germany (26 Jan 2015)
Paul S, Janz D, Polle A (2013) The effect of drought stress on growth and cytokinin responsiveness of Grey Poplar (Populus × canescens) transformed with an ARR5::GUS construct. Forstbotanisches Seminar, Department of Forest Botany and Tree Physiology, University of Göttingen, Germany (21 Jan 2013)
This project is funded by a Ph.D. scholarship granted to S.P. by the European Commission in the Erasmus Mundus (India4EU II) program and by WATBIO funded from the European Union's Seventh Programme for research, technological development and demonstration under grant agreement No. FP7- 311929.