Development of microsatellites in Prosopis spp. and their application to study the reproduction system
Abstract:
Prosopis chilensis and P. flexuosa (Fabaceae) are closely related hardwood arboreal species that are widely distributed in the arid regions of Argentina. The development of highly polymorphic markers, such as microsatellites, is desirable for genetic studies of these species. Here we present the development and characterization of six polymorphic microsatellite markers in Prosopis chilensis and P. flexuosa. These markers showed a Polymorphic Information Content between 0.14 and 0.85 and the number of alleles varied from 2 to 13 considering both species. They also revealed a broad cross-species affinity when tested in seven other Prosopis species. All the markers amplified in at least 5 species.
Mottura, M.C., Gailing, O., Verga, R.A. & R. Finkeldey. 2004. Efficiency of microsatellite enrichment in Prosopis chilensis using magnetic capture. Plant Molecular Biology Reporter 22: 251-258.
Mottura, M.C., Finkeldey, R., Verga, R.A. & O. Gailing. 2005. Development and characterization of microsatellite markers for Prosopis chilensis and P. flexuosa and cross-species amplification. Molecular Ecology Notes 5: 487-489.