Kramer, Wilfried, PD Dr.

Privatdozent Molecular Biology and Genetics

  • Diploma (Biology), University of Cologne, Germany, 1982
  • Dr. rer. nat., University of Cologne, Germany, 1986
  • Postdoctoral Fellow, University of California, Berkeley, USA, 1986 - 1989
  • Habilitation in Molecular Biology and Genetics, University of Göttingen, Germany, 2000
  • At the Dept. of Molecular Genetics since 1989

Major Research Interests

Working in the Department of Molecular Genetics, which is headed by Prof. Dr. H. Krebber, my major scientific interest is focused at present on the interplay of RNA metabolism with other cellular pathways, namely DNA replication/DNA repair and cell cycle/cell division. There is strong genetic evidence that the RNA-binding SR-protein Npl3 from budding yeast, which is involved in many RNA-related cellular processes, also plays an important role in the maintenance of genome stability. We want to further understand the connections between these seemingly unrelated processes. One clue comes from the finding of other labs that RNA-DNA hybrids, so called R-loops, can induce DNA damage and homologous recombination. We try to find out, where in this process Npl3 might be involved, combining genetical and biochemical approaches.

Homepage Department/Research Group

Selected Recent Publications

  • Zander G, Kramer W, Seel A and Krebber H (2017) Saccharomyces cerevisiae Gle2/Rae1 is involved in septin organization, essential for cell cycle progression. Yeast 34: 459-470.
  • Popova B, Schubert S, Bulla I, Buchwald D and Kramer W (2015) A robust and versatile method for combinatorial chemical synthesis of gene libraries via hierarchical assembly of partially randomized modules. PLoS One 10: e0136778.
  • Ede C, Rudolph CJ, Lehmann S, Schürer KA, Kramer W (2011) Budding yeast Mph1 promotes sister chromatid interactions by a mechanism involving strand invasion. DNA Repair 10, 45-55.
  • Schomacher L, Schürer KA, Ciirdaeva E, McDermott P, Chong J, Kramer W, Fritz HJ (2010) Archaeal DNA uracil repair via direct strand incision: A minimal system reconstituted from purified components. DNA Repair 9, 438-447.
  • Panico ER, Ede C, Schildmann M, Schürer KA, Kramer W (2010) Genetic evidence for a role of Saccharomyces cerevisiae Mph1 in recombinational repair under replicative stress. Yeast 27, 11-27.
  • Prakash R, Satory D, Dray E, Papusha A, Scheller J, Kramer W, Krejci L, Klein H, Haber JE, Sung P, Ira G (2009) Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Genes Dev 23, 67-79.
  • Schürer KA, Rudolph C, Ulrich HD, Kramer W (2004) Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from homologous recombination, but not from postreplication repair. Genetics 166, 1673-1686.