Urlaub, Henning, Prof. Dr.
Group Leader - Bioanalytical Mass Spectrometry Groupfrom 2010: Group leader “Bioanalytical Mass Spectrometry” group at the Max Planck Institute for Biophysical Chemistry, Göttingen and “Bioanalytics” group at University Medical Center Göttingen (UMG) within Dept. of Clinical Chemistry
2010: Professor at the Faculty of Medicine at Georg August University Göttingen
2005: Research group “Bioanalytical Mass Spectrometry Group” at the Max Planck Institute for Biophysical Chemistry
2001: Responsibility for running the mass spectrometry unit in the Dept. of Cellular Biochemistry at the Max Planck Institute for Biophysical Chemistry in Göttingen
2000–2001: Guest researcher at the EMBL in Heidelberg, Germany, in the group of Dr. Matthias Wilm
1997–2001: Post-Doc at the "Institut für Molekularbiologie und Tumorforschung" (IMT) of the Philipps University of Marburg, Germany (Group of Reinhard Lührmann) and at the Max Planck Institute for Biophysical Chemistry in Göttingen (Group of Reinhard Lührmann)
1993–1996 Ph.D. and Post-Doc in the research group of Prof. Brigitte Wittmann-Liebold at the Max Delbrück Center for Molecular Medicine (MDC) in Berlin
1992–1993 Diploma thesis in the research group of Prof. Volker A. Erdmann at the Institute of Biochemistry of the Free University of Berlin
1987–1993 Studied biochemistry at the Free University of Berlin, Germany
Major Research Interests
Modern mass-spectrometric methods have become key technologies in the life sciences. We apply “state-of-the-art” mass spectrometry to elucidate quantitative changes of proteins and their post-translational modifications derived from different samples, including tissue, cells, organelles, and cell compartments. In addition, we apply mass spectrometric (MS) methods to monitor dynamic changes of protein and protein-ligand complexes through use of cross-linking. Following main projects are investigated by the use of MS are: 1. Monitoring protein abundance, modifications and interactions in the non-stimulated and stimulated synapse by MS, 2. Protein-protein cross-linking combined with MS in stimulated and resting B cells, 3. Method development in protein-protein, protein-RNA and protein-DNA cross-linking combined with MS.
Homepage Department/Research Group
Selected Recent Publications
Fornasiero EF, Mandad S, …, Urlaub H*, Rizzoli S (2018) Precisely measured protein lifetimes in the mouse brain reveals biologically-significant differences across tissues and subcellular fractions, Nature Communications (*co-corresponding author)
Vos SM, Farnung L, Boehning M, Wigge C, Linden A, Urlaub H, Cramer P (2018) Structure of activated transcription complex Pol II-DSIF-PAF-SPT6. Nature, 560, 607-612.
Schmidt C, Urlaub H (2017) Combining cryo-electron microscopy (cryo-EM) and cross-linking mass spectrometry (CX-MS) for structural elucidation of large protein assemblies. Curr Opin Struct Biol. 46, 157-168.
Pan KT, Chen CC, Urlaub H*, Khoo KH (2017) Adapting Data-Independent Acquisition for Mass Spectrometry-Based Protein Site-Specific N-Glycosylation Analysis. Anal Chem., 89, 4532-4539. (*co-corresponding author)
Agafonov DE, Kastner B, Dybkov O, Hofele RV, Liu WT, Urlaub H*, Lührmann R, Stark H. (2016) Molecular architecture of the human U4/U6.U5 tri-snRNP. Science, 351, 1416-1420. (*co-corresponding author)
Corso J, Pan KT, Walter R, Doebele C, Mohr S, Bohnenberger H, Ströbel P, Lenz C, Slabicki M, Hüllein J, Comoglio F, Rieger MA, Zenz T, Wienands J, Engelke M, Serve H, Urlaub H*, Oellerich T. (2016) Elucidation of tonic and activated B-cell receptor signaling in Burkitt's lymphoma provides insights into regulation of cell survival. Proc Natl Acad Sci U S A, 113, 5688-5693. (*co-corresponding author)
Kırlı K, Karaca S, Dehne HJ, Samwer M, Pan KT, Lenz C, Urlaub H*, Görlich D. (2015) A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning. Elife, 4. pii: e11466 (* co-corresponding author)
Wilhelm, B. G., Mandad, S., Truckenbrodt, S., Krohnert, K., Schafer, C., Rammner, B., Koo, S. J., Classen, G. A., Krauss, M., Haucke, V., Urlaub, H., Rizzoli, S. O. (2014). Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. Science, 344, 1023-1028.
Kramer K, Sachsenberg T, Beckmann BM, Qamar S, Boon KL, Hentze MW, Kohlbacher O, Urlaub H (2014) Photo-cross-linking and high-resolution mass spectrometry for assignment of RNA-binding sites in RNA-binding proteins. Nature Methods 11: 1064-1070.