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M.Cp.0019 Basic Laboratory Methods (3ECTS)

BASIC LABORATORY TECHNIQUES

Coordinator
Prof. P. Karlovsky, Department of Crop Science, Division of Molecular Phytopathology and Mycotoxine Research

Language
English

Type of instruction/contact hours
Practical 14 h and lecture 14 h

Frequency (WS/SS) and duration
WS and SS, one semester

Instructors
Dr.A.Sirrenberg

Contents and objectives
This course aims at preparing students for practical research, including basic chemical and microbiological techniques as well as responsible behaviour in the laboratory and operating of machines. It includes laboratory safety, planning and recording of experiments, chemical calculations (concentrations in media and buffer), sterilization techniques, working in sterile conditions, pH, photometry, ELISA, PCR. Preparation of experiments and writing protocols.
Safe and responsible behavior and working in the laboratory is a prerequisite for an experimental MSc thesis in Phytopathology. Students learn to plan and execute safely experiments, to explain and use methods and equipment effectively..

Exam Requirements:
Basic knowledge of calculation of concentrations,
sterilization techniques, importance and composition of buffers, principles of
Photometry, centrifugation, ELISA and PCR.

Exam requirements
Accepted protocol

Examination
Written test: 45 minutes

Number of students
10