A01 - isoSTED microscopy for live cell imaging

The aim of this project is to expand isoSTED microscopy to imaging under strongly aberrated imaging conditions, such as those introduced by thick biological tissue. By introducing an adaptive optics based aberration correction system it will be possible to achieve resolutions similar to those obtained nowadays under optimized imaging conditions. In addition, we intend to make the microscope available the other projects of the SFB and to explore the feasibility of isoSTED FCS essays.

Members of this project:
Dr. Alexander Egner
Dipl. Phys. René Siegmund


Böhm, U., Hell, S. W. and Schmidt, R. (2016)
4Pi-RESOLFT nanoscopy
NATURE COMMUNICATION, DOI:DOI: 10.1038/ncomms10504

Aquino, D., Schönle, A., Geisler, C., Middendorff, C. V., Wurm, C. a., Okamura, Y., Lang, T., Hell, S. W. and Egner, A. (2011)
Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores.
Nat. Methods, 8(4): 353-9, DOI:10.1038/nmeth.1583

Hua, Y., Sinha, R., Thiel, C. S., Schmidt, R., Hüve, J., Martens, H., Hell, S. W., Egner, A. and Klingauf, J. (2011)
A readily retrievable pool of synaptic vesicles.
Nat. Neurosci.Nature Publishing Group,, 14(7): 833-9, DOI:10.1038/nn.2838

Okamura, Y., Schmidt, R., Raschke, I., Hintze, M., Takeoka, S., Egner, A. and Lang, T. (2011)
A few immobilized thrombins are sufficient for platelet spreading.
Biophys. J.Biophysical Society,, 100(8): 1855-63, DOI:10.1016/j.bpj.2011.02.052

Ullal, C. K., Primpke, S., Schmidt, R., Böhm, U., Egner, A., Vana, P. and Hell, S. W. (2011)
Flexible Microdomain Specific Staining of Block Copolymers for 3D Optical Nanoscopy
Macromolecules, 44(19): 7508-7510, DOI:10.1021/ma201504f

Hell, S. W., Schmidt, R. and Egner, A. (2009)
Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses
Nat. PhotonicsNature Publishing Group,, 3(7): 381-387, DOI:10.1038/nphoton.2009.112

Schmidt, R., Wurm, C. a., Punge, A., Egner, A., Jakobs, S. and Hell, S. W. (2009)
Mitochondrial cristae revealed with focused light
Nano Lett., 9(6): 2508-10, DOI:10.1021/nl901398t

Ullal, C. K., Schmidt, R., Hell, S. W. and Egner, A. (2009)
Block copolymer nanostructures mapped by far-field optics.
Nano Lett., 9(6): 2497-2500, DOI:10.1021/nl901378e

Schmidt, R., Wurm, C. a., Jakobs, S., Engelhardt, J., Egner, A. and Hell, S. W. (2008)
Spherical nanosized focal spot unravels the interior of cells
Nat. Methods, 5(6): 539-44, DOI:10.1038/nmeth.1214