B11 - Nanoscale observations of membrane dynamics
The lipid composition of biological membranes, along with its spatial organization and temporal dynamics, are acknowledged to be fundamental to cellular and protein functionality. However, techniques for observing membrane heterogeneity are often limited in terms of spatial and temporal resolution or influence on the system under study. Here, we use the superior spatio-temporal resolution of STED microscopy and fast single-molecule tracking to determine how molecular interactions of the membrane may coalesce into signaling platforms.
Members of this project:
Prof. Dr. Stefan W. Hell
Dr. Christian Eggeling
Dr. Roman Schmidt
Débora Machado Andrade
Honigmann, A., Mueller, V., Ta, H., Schoenle, A., Sezgin, E., Hell, S. W. and Eggeling, C. (2014)
Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells.
Nat. Commun.Nature Publishing Group,, 5: 5412, DOI:10.1038/ncomms6412