A 3 - High-resolution stress-field mapping in fiber networks and cells

Generation and transmission of forces and stresses are essential in many cellular processes and cells are structurally complex and inhomogeneous. This project aims at measuring locally resolved internal stress fields in model networks using self-assembled FRET force sensors based on DNA. The experimental endeavor is complemented with numerical modeling of increasingly complex and heterogeneous rigid, semiflexible and flexible polymer assemblies, relying on novel and highly efficient methods for physically simulating viscoelastic materials made of slender filaments.

Members of this project:

Prof. Dr. Christoph F. Schmidt
Dr. Florian Rehfeldt
Prof. Dr. Max Wardetzky
M.Sc. Christina Jayachandra


Heidemann, K. M., Sageman-Furnas, A. O., Sharma, A., Rehfeldt, F., Schmidt, C. F. and Wardetzky, M. (2018)
Topology determines force distributions in one-dimensional random spring networks
Phys. Rev. E, 97: 022306

Heidemann, K., Sageman-Furnas, A. O., Sharma, A., Rehfeldt, F., Schmidt, C. and Wardetzky, M. (2018)
Topology counts: force distributions in circular spring networks
Phys. Rev. Lett., 120: 068001, DOI:10.1103/PhysRevLett.120.068001

Rehfeldt, F. and Schmidt, C. (2017)
Physical probing of cells
J. Phys. D: Appl. Phys., 50: 463001, DOI:doi.org/10.1088/1361-6463/aa8aa6

Prabhune, M., Rehfeldt, F. and Schmidt, C. (2017)
Molecular force sensors to measure stress in cells
Journal of Physics D: Applied Physics, 50(23): 233001

Prabhune, M., von Roden, K., Rehfeldt, F. and Schmidt, C. F. (2016)
Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro
PLoS ONE, 11, DOI:10.1371/journal.pone.0161623

Heidemann, K.M., Sharma, A., Rehfeldt, F., Schmidt, C. F. and Wardetzky, M. (2015)
Elasticity of 3D networks with rigid filaments and compliant crosslinks
Soft Matter, 11: 343-354, DOI:10.1039/C4SM01789G

Kaliman, S., Jayachandran, C., Rehfeldt, F. and Smith, A.-S. (2014)
Novel growth regime of MDCK II model tissues on soft substrates.
Biophys. J., 106(7): L25-8, DOI:10.1016/j.bpj.2013.12.056

Sharma, A., Sheinman, M., Heidemann, K. M. and MacKintosh, F. C. (2013)
Elastic response of filamentous networks with compliant crosslinks
Phys. Rev. E, 88(5): 052705, DOI:10.1103/PhysRevE.88.052705

Bremerich, M. (2012)
High-bandwidth microrheology of cytoskeletal networks
Georg-August-University Göttingen,

Nijenhuis, N., Mizuno, D., Spaan, J. A. E. and Schmidt, C. F. (2012)
High-resolution microrheology in the pericellular matrix of prostate cancer cells.
J. R. Soc. Interface, 9(73): 1733-44, DOI:10.1098/rsif.2011.0825

MacKintosh, F. C. and Schmidt, C. F. (2010)
Active cellular materials
Cell Biology, 22(1): 29?35, DOI:doi:10.1016/j.ceb.2010.01.002

Mizuno, D., Bacabac, R., Tardin, C., Head, D. and Schmidt, C. (2009)
High-resolution probing of cellular force transmission
Phys. Rev. Lett., 102

Nijenhuis, N., Mizuno, D., Spaan, J. A. E. and Schmidt, C. F. (2009)
Viscoelastic response of a model endothelial glycocalyx
Physical Biology, 6(2): 025014, DOI:doi:10.1088/1478-3975/6/2/025014

Atakhorrami, M., Mizuno, D., Koenderink, G. H., Liverpool, T. B., MacKintosh, F. C. and Schmidt, C. F. (2008)
Short-time inertial response of viscoelastic fluids measured with Brownian motion and with active probes
Phys. Rev. E, 77(6): 061508, DOI:10.1103/PhysRevE.77.061508

Mizuno, D., Head, D. A., MacKintosh, F. C. and Schmidt, C. F. (2008)
Active and passive microrheology in equilibrium and nonequilibrium systems
Macromolecules, 41: 7194-7202, DOI:10.1021/ma801218z

Jabbari-Farouji, S., Mizuno, D., Derks, D., Wegdam, G. H., MacKintosh, F. C., Schmidt, C. F. and Bonn, D. (2008)
Effective temperatures from the fluctuation-dissipation measurements in soft glassy materials
Euro Phys. Lett., 84: 6, DOI:10.1209/0295-5075/84/20006

Nijenhuis, N., Mizuno, D., Schmidt, C. F., Vink, H. and Spaan, J. A. E. (2008)
Microrheology of hyaluronan solutions: Implications for the endothelial glycocalyx
Biomacromolecules, 9: 2390-2398, DOI:10.1021/bm800381z

Walder, R., Schmidt, C. F. and Dennin, D. (2008)
Combined macro- and microrheometer for use with Langmuir monolayers
Review of Scientific Instruments, 79(6): 063905, DOI:10.1063/1.2937649