B 7 - Dynamics of intermediate filament self-assembly

Intermediate filaments in the cytoskeleton of eukaryotes are a major contributor to the mechanical properties of cells. The hierarchical build-up of the filaments from subunits and the formation of networks will be studied. We will combine nano- and micro-imaging and scattering methods with microfluidic techniques. In particular, our aim is to understand the influence of mono- or multivalent ions on the assembly and the resulting mechanical properties.

Members of this project:

Prof. Dr. Sarah Köster
Johanna Block
Michaela Denz


Brennich, M., Vainio, U., Wedig, T., Bauch, S., H., H. and Köster, S. (2019)
Mutation-induced alterations of intra-filament subunit organization in vimentin filaments revealed by SAXS
Soft Matter, 15: 1999-2008, DOI:10.1039/c8sm02281j

Lopez, C. G., Saldanha, O., Aufderhorst-Roberts, A., Martinez-Torres, C., Kuijs, M., Koenderink, G. H., Köster*, S., and Huber*, K. (*corresponding authors) (2018)
Effect of ionic strength on the structure and elongational kinetics of vimentin filaments
Soft Matter, DOI:10.1039/C8SM01007B

Block, J., Witt, H., Candelli, A., Cabanas Danes, J., Peterman, E. J. G., Wuite, G. J. L., Janshoff, A. and Köster, S. (2018)
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Science Advances, 4(5): eaat1161, DOI:10.1126/sciadv.aat1161

Denz, M., Brehm, G., Hémonnot, C. Y. J., Spears, H., Wittmeier, A., Cassini, C., Saldanha, O., Perego, E., Diaz, A., Burghammer, M. and Köster, S. (2018)
Cyclic olefin copolymer as an X-ray compatible material for microfluidic devices
Lab on a Chip, 18: 171 - 178, DOI:10.1039/c7lc00824d

Block, J., Witt, H., Candelli, A., Peterman, E. J. G., Wuite, G. J. L., Janshoff, A. and Köster, S. (2017)
Nonlinear Loading-Rate-Dependent Force Response of Individual Vimentin Intermediate Filaments to Applied Strain
PHYSICAL REVIEW LETTERS, 118: 048101, DOI:org/10.1103/PhysRevLett.118.048101

Saldanha, O., Brennich, M. E., Burghammer, M., Herrmann, H. and Köster, S. (2016)
The filament forming reactions of vimentin tetramers studied in a serial-inlet microflow device by small angle x-ray scattering
AIP Biomicrofluidics, 10: 024108, DOI:10.1063/1.4943916

Lopez, C. G., Saldanha, O., Huber, K. and Köster, S. (2016)
Lateral association and elongation of vimentin intermediate filament proteins: A time-resolved light-scattering study
PNAS, 113(40): 11152 ? 11157, DOI:10.1073/pnas.1606372113

Block, J., Schroeder, V., Pawelzyk, P., Willenbacher, N. and Köster, S. (2015)
Physical properties of cytoplasmic intermediate filaments
ELSEVIER, 1853: 3053, DOI:doi:10.1016/j.bbamcr.2015.05.009

Dammann, C., Herrmann, H. and Köster, S. (accepted for publication)
Competitive counterion binding regulates the aggregation onset of vimentin intermediate filaments
Israel Journal of Chemistry: accepted for publication

Brennich, M. E., Bauch, S., Vainio, U., Wedig, T., Herrmann, H. and Köster, S. (2014)
Impact of ion valency on the assembly of vimentin studied by quantitative small angle X-ray scattering
Soft Matter, 10(12): 2059, DOI:10.1039/c3sm52532e

Dammann, C. and Köster, S. (2014)
Dynamics of counterion-induced attraction between vimentin filaments followed in microfluidic drops.
Lab Chip, 14(15): 2681-7, DOI:10.1039/c3lc51418h

Nöding, B., Herrmann, H. and Köster, S. (2014)
Direct Observation of Subunit Exchange Along Mature Vimentin Intermediate Filaments
Biophysical Journalopen access,: 2923-2931, DOI:10.1016/j.bpj.2014.09.050

Brennich, M. E. and Köster, S. (2013)
Tracking reactions in microflow
Microfluid. Nanofluidics, 16(1-2): 39-45, DOI:10.1007/s10404-013-1212-y

Dammann, C., Nöding, B. and Köster, S. (2012)
Vimentin networks at tunable ion-concentration in microfluidic drops.
Biomicrofluidics, 6(2): 22009-2200910, DOI:10.1063/1.4705103

Köster, S. and Pfohl, T. (2012)
x-ray studies of biological matter in microfluidic environments
Mod. Phys. Lett. B, 26(26): 1230018, DOI:10.1142/S0217984912300189

Köster, S., Lin, Y.-C., Herrmann, H. and Weitz, D. a. (2010)
Nanomechanics of vimentin intermediate filament networks
Soft Matter, 6(9): 1910, DOI:10.1039/c000113a

Nöding, B. and Köster, S. (2012)
Intermediate Filaments in Small Configuration Spaces
Phys. Rev. Lett., 108(8): 088101, DOI:10.1103/PhysRevLett.108.088101

Brennich, M. E., Nolting, J.-F., Dammann, C., Nöding, B., Bauch, S., Herrmann, H., Pfohl, T. and Köster, S. (2011)
Dynamics of intermediate filament assembly followed in micro-flow by small angle X-ray scattering.
Lab Chip, 11(4): 708-16, DOI:10.1039/c0lc00319k

Priebe, M., Kalbfleisch, S., Tolkiehn, M., Köster, S., Abel, B., Davies, R. J. and Salditt, T. (2010)
Orientation of biomolecular assemblies in a microfluidic jet
New J. Phys.open access,, 12(4): 043056, DOI:10.1088/1367-2630/12/4/043056