B10 - Structure-dynamics-function in photoswitchable proteins

Targeting the very short and short living structures of the various intermediates formed in reversibly photoswitchable fluorescent proteins during their photo life cycle is the major aim of project B10. Technically, we will face this ambitious goal by applying novel and state of the art time-resolved x-ray scattering and time-resolved x-ray diffraction experiments at synchrotron and FEL sources. We will utilize the structural dynamics data to develop novel and optimized variants of reversibly photoswitchable fluorescent proteins for applications in super-resolution microscopy.

Members of this project:

Prof. Dr. Stefan Jakobs
Prof. Dr. Simone Techert
Krystof Idzik


Frahm, L., Keller-Findeisen, J., Alt, P., Schnorrenberg, S., del Álamo Ruiz, M., Aspelmeier, T., Munk, A., Jakobs, S. and Hell, S. (2019)
Molecular contribution function in RESOLFT nanoscopy
Opt. Express, 27(15): 21956, DOI:org/10.1364/OE.27.021956

Kamper, M., Ta, H., Jensen, N. A., Hell, S. W. and Jakobs, S. (2018)
Near-infrared STED nanoscopy with an engineered bacterial phytochrome
NATURE COMMUNICATIONS, 9(4762): 1-7, DOI:10.1038/s41467-018-07246-2

Döring, F., Marschall, F., Yin, Z., Rösner, B., Beye, M., Miedema, P., Kubiček, K., Glaser, L., Raiser, D., Soltau, J., Guzenko, V., Viefhaus, J., Buck, J., Risch, M., Techert, S. and David, C. (2018)
1D-Full Field Microscopy of Elastic and Inelastic Scattering with Transmission off-axis Fresnel Zone Plates
Proc. Int. X-Ray Microscop. Conf., 24: 184-185, DOI:10.1017/S1431927618013260

David, C., Rösner, B., Döring, F., Guzenko, V., Koch, F., Lebugle, M., Marschall, F., Seniutinas, G., Raabe, J., Watts, B., Grolimund, D., Yin, Z., Beye, M., Techert, S., Viefhaus, J., Falkenberg, G. and Schroer, C. (B03, B10)
Diffractive X-ray Optics for Synchrotrons and Free -Electron Lasers
Microsc. Microanal., 24: 264, DOI:10.1017/S1431927618013673

Bari, S., Egorov, D., Jansen, T. L. C., Boll, R., Hoekstra, R., Techert, S., Zamudio‐Bayer, V., Bülow, C., Lindblad, R., Leistner, G., Ławicki, A., Hirsch, K., Miedema, P. S., von Issendorff, B., Lau, J. T. and Schlathölter, T. (2018)
Soft X‐ray Spectroscopy as a Probe for Gas‐Phase Protein Structure: Electron Impact Ionization from Within
Chem. Europ. J., 24(30), DOI:10.1002/chem.201801440

Yin, Z., Inhester, L., Thekku Veedu, S., Quevedo, W., Pietzsch, A., Wernet, P., Groenhof, G., Föhlisch, A., Grubmüller, H. and Techert, S. (2017)
Cationic and Anionic Impact on the Electronic Structure of Liquid Water
J. Phys. Chem. Lett., 8: 3759−3764, DOI:10.1021/acs.jpclett.7b01392

Kunnus, K., Josefsson, I., Rajkovic, I., Quevedo, W., Beye, M., Weniger, C. and Grübel, S. (2016)
Identification of the dominant photochemical pathways and mechanistic insights to the ultrafast ligand exchange of Fe(CO)5 to Fe(CO)4EtOH
STRUCTURAL DYNAMICS, 3(3): 043204, DOI:10.1063/1.4941602

Jain, R., Khan, N., Menzel, A., Rajkovic, I., Konrad, M. and Techert, S. (2016)
Insights into open/closed conformations of the catalytically active human guanylate kinase as investigated by small-angle X-ray scattering.
Eur Biophys J, 45(1), DOI:10.1007/s00249-015-1079-9

Jensen, N. a., Danzl, J. G., Willig, K. I., Lavoie-Cardinal, F., Brakemann, T., Hell, S. W. and Jakobs, S. (2014)
Coordinate-targeted and coordinate-stochastic super-resolution microscopy with the reversibly switchable fluorescent protein dreiklang.
Chemphyschem, 15(4): 756-62, DOI:10.1002/cphc.201301034

Thekku Veedu, S., Raiser, D., Kia, R., Scholz, M. and Techert, S. (2014)
Ultrafast dynamical study of pyrene-N,N-dimethylaniline (PyDMA) as an organic molecular diode in solid state.
J. Phys. Chem. B, 118(12): 3291-7, DOI:10.1021/jp4121222

Thekku Veedu, S., Scholz, M., Kia, R., Paulmann, C. and Techert, S. (2014)
Acta Crystallogr. Sect. E. Struct. Rep. OnlineInternational Union of Crystallography,, 70(Pt 1): o16, DOI:10.1107/S1600536813032698

Yin, Z., Rajkovic, I., Kubicek, K., Quevedo, W., Pietzsch, A., Wernet, P., Föhlisch, A. and Techert, S. (2014)
Probing the Hofmeister effect with ultrafast core-hole spectroscopy.
J. Phys. Chem. B, 118(31): 9398-403, DOI:10.1021/jp504577a

Boll, R., Anielski, D., Bostedt, C., Bozek, J., Christensen, L., Coffee, R., De, S., Decleva, P., Epp, S., Erk, B., Foucar, L., Krasniqi, F., Küpper, J., Rouzée, A., Rudek, B., Rudenko, A., Schorb, S., Stapelfeldt, H., Stener, M., Stern, S., Techert, S., Trippel, S., Vrakking, M., Ullrich, J. and Rolles, D. (2013)
Femtosecond photoelectron diffraction on laser-aligned molecules: Towards time-resolved imaging of molecular structure
Phys. Rev. A, 88(6): 061402, DOI:10.1103/PhysRevA.88.061402

Mitzner, R., Rehanek, J., Kern, J., Gul, S., Hattne, J., Taguchi, T., Alonso-Mori, R., Tran, R., Weniger, C., Schröder, H., Quevedo, W., Laksmono, H., Sierra, R. G., Han, G., Lassalle-Kaiser, B., Koroidov, S., Kubicek, K., Schreck, S., Kunnus, K., Brzhezinskaya, M., Firsov, A., Minitti, M. P., Turner, J. J., Moeller, S., Sauter, N. K., Bogan, M. J., Nordlund, D., Schlotter, W. F., Messinger, J., Borovik, A., Techert, S., de Groot, F. M. F., Föhlisch, A., Erko, A., Bergmann, U., Yachandra, V. K., Wernet, P. and Yano, J. (2013)
L-Edge X-ray Absorption Spectroscopy of Dilute Systems Relevant to Metalloproteins Using an X-ray Free-Electron Laser.
J. Phys. Chem. Lett., 4(21): 3641-3647, DOI:10.1021/jz401837f

Simao, C., Mas-Torrent, M., André, V., Teresa Duarte, M., Techert, S., Veciana, J. and Rovira, C. (2013)
Solid state photodimerisation of tetrathiafulvalene derivatives bearing carboxylate and carboxylic acid substituents
CrystEngComm, 15(46): 9878, DOI:10.1039/c3ce41353e

Yin, Z., Rajković, I., Raiser, D., Scholz, M. and Techert, S. (2013)
Experimental setup for high resolution x-ray spectroscopy of solids and liquid samples
Klisnick, A. & Menoni, C. S.X-Ray Lasers Coherent X-Ray Sources Dev. Appl. X, 8849: 88490I, DOI:10.1117/12.2023992

Grotjohann, T., Testa, I., Reuss, M., Brakemann, T., Eggeling, C., Hell, S. W. and Jakobs, S. (2012)
rsEGFP2 enables fast RESOLFT nanoscopy of living cells.
Elife, 1: e00248, DOI:10.7554/eLife.00248