C 1 - 3D density distribution of cells by x-ray holotomography

The project will significantly enhance holographic x-ray imaging and tomography. The goals are: (i) optimized phase retrieval with less idealizations, (ii) higher dimensionality, in particular by generalisation to time-resolved imaging, (iii) an extension from synchrotron-based phase contrast imaging to compact laboratory sources, and (iv) closing the gap from proof-of-concept to applications on cells and tissue.

Members of this project:

Prof. Dr. Tim Salditt
Marina Eckermann

Associated members of this project:


Töpperwien, M., Markus, A., Alves, F. and Salditt, T. (2019)
Contrast enhancement for visualizing neuronal cytoarchitecture bypropagation-based x-ray phase-contrast tomography
NeuroImage, 199: 70-80, DOI:10.1016/j.neuroimage.2019.05.043

Ruhlandt, A. and Salditt, T. (2019)
Time-resolved x-ray phase-contrast tomography of sedimenting micro-spheres
New J. Phys., 21: 043017, DOI:10.1088/1367-2630/ab13c8

Toepperwien, M., Doeppner, T. R., Zechmeister, B., Bähr, M. and Salditt, T. (2019)
Multiscale x-ray phase-contrast tomography in a mouse model of transient focal cerebral ischemia
BIOMEDICAL OPTICS EXPRESS, 92: 338372, DOI:10.1364/BOE.10.000092

Hagemann, J., Töpperwien, M. and Salditt, T. (2018)
Phase retrieval for near-field X-ray imaging beyond linearisation or compact support
Appl. Phys. Lett., 113: 041109, DOI:10.1063/1.5029927

Töpperwien, M., van der Meer, F., Stadelmann, C. and Salditt, T. (2018)
Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography
PNAS, DOI:10.1073/pnas.1801678115

Hagemann, J. and Salditt, T. (2018)
Coherence-resolution relationship in holographic and coherent diffractive imaging
Opt. Express, 26: 242-253

Lohse, L. M., Vassholz, M. and Salditt, T. (2017)
Tomography with extended sources: Theory, error estimates, and a reconstruction algorithm
PHYSICAL REVIEW A, 96: 063804, DOI:10.1103/PhysRevA.96.063804

Krenkel, M., Toepperwien, M., Alves, F. and Salditt, T. (2017)
Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime
Journal of Applied Crystallography, 73: 282-292, DOI:10.1107/S2053273317007902

Jahn, T., Wilke, R., Chushkin, Y. and Salditt, T. (2017)
How many photons are needed to reconstruct random objects in coherent X-ray diffractive imaging?
Acta Cryst., 73: 2053-2733, DOI:doi.org/10.1107/S20532733160

Hagemann, J., Robisch, A.-L., Osterhoff M. and Salditt, T. (2017)
Probe reconstruction for holographic X-ray imaging
J. Synchrotron Rad., 24: 498-505, DOI:org/10.1107/S160057751700128

Töpperwien, M., Krenkel, M., Vincenz, D., Stöber, F., Oelschlegel, A. M., Goldschmidt J. and Salditt, T. (2017)
Three-dimensional mouse brain cytoarchitecture revealed by laboratory-based x-ray phase-contrast tomography
Sci. Rep., 7: 42847, DOI:10.1038/srep42847

Pein, A., Loock, S., Plonka, G. and Salditt, T. (2016)
Using sparsity information for iterative phase retrieval in x-ray propagation imaging
Optics Express, 24(8): pp. 8332-8343, DOI:10.1364/OE.24.008332

Töpperwien, M., Krenkel, M., Quade, F. and Salditt, T. (2016)
Laboratory-based x-ray phase-contrast tomography enables 3D virtual histology
Proc. SPIE 9964, Advances in Laboratory-based X-Ray Sources, Optics, and Applications V, 99640I, DOI:10.1117/12.2246460

Töpperwien, M., Krenkel, M., Müller, K. and Salditt, T. (2016)
Phase-contrast tomography of neuronal tissues: from laboratory- to high resolution synchrotron CT
Proc. SPIE 9967, Developments in X-Ray Tomography X, 99670T, DOI:10.1117/12.2238496

Kramer, S. C., Hagemann, J., Künneke, L. and Lebert, J. (2016)
Parallel Statistical Multiresolution Estimation for Image Reconstruction
SIAM Journal on Scientific Computing, 38(5): C533-C559, DOI:10.1137/15M1020332

Wallentin, J., Osterhoff, M. and Salditt, T. (2016)
In Operando X-Ray Nanodiffraction Reveals Electrically Induced Bending and Lattice Contraction in a Single Nanowire Device
Advanced materials, 28: 1788-1792, DOI:10.1002/adma.201504188

Krenkel, M., Töpperwien, M., Dullin, C., Alves, F. and Salditt, T. (2016)
Propagation-based phase-contrast tomography for high-resolution lung imaging with laboratory sources
AIP Advances, 6: 035007, DOI:10.1063/1.4943898

Vassholz, M., Koberstein-Schwarz, B., Ruhlandt, A., Krenkel, M. and Salditt, T. (2016)
New X-Ray Tomography Method Based on the 3D Radon Transform Compatible with Anisotropic Sources

Bernhardt, M., Priebe, M., Osterhoff, M., Wollnik, C., Diaz, A., Salditt, T. and Rehfeldt, F. (2016)
X-Ray Micro- and Nanodiffraction Imaging on Human Mesenchymal Stem
Cells and Differentiated Cells

Biophysical Journal, 110(3): 680-690, DOI:10.1016/j.bpj.2015.12.017

T. Salditt, Hoffmann, S., Vassholz, M., Haber, J., Osterhoff, M. and Hilhorst, J. (2015)
X-Ray Optics on a Chip: Guiding X Rays in Curved Channels
Physical Review Letters, 115: 203902, DOI:10.1103/PhysRevLett115.203902

Robisch, A.-L., Kröger, K., Rack, A. and Salditt, T. (2015)
Near-field ptychography using lateral and longitudinal shifts
New J. Phys., 17: 073033, DOI:10.1088/1367-2630/17/7/073033

Chen, H.-Y., Hoffmann, S. and Salditt, T. (2015)
X-ray beam compression by tapered waveguides
Appl. Phys. Lett., 106: 194105, DOI:10.1063/1.4921095

Maretzke, S. (2015)
A uniqueness result for propagation-based phase contrast imaging from a single measurement
Inverse Problems, 31: 065003, DOI:http://dx.doi.org/10.1088/0266-5611/31/6/065003

Martin Krenkel Andrea Markus, M. B. C. D. F. A. and Salditt, T. (2015)
Phase-contrast zoom tomography reveals precise locations of macrophages in mouse lungs
Scientific Reports, 5:09973, DOI:10.1038/srep09973

Homann, C., Hohage, T., Hagemann, J., Robisch, A. and Salditt, T. (2015)
Validity of the empty-beam correction in near-field imaging
Phys. Rev. A, 91: 013821, DOI:10.1103/PhysRevA.91.013821

Bartels, M., Krenkel, M., Haber, J., Wilke R. N. and Salditt, T. (2015)
X-Ray Holographic Imaging of Hydrated Biological Cells in Solution
Phys. Rev. Lett., 114(4): 048103, DOI:10.1103/PhysRevLett.114.048103

Hagemann, J., Robisch, A. and Luke, D. (2014)
Reconstruction of wave front and object for inline holography from a set of detection planes
Opt. Express, 22(10): 195-202, DOI:10.1364/OE.22.011552

Ruhlandt, A., Krenkel, M., Bartels, M. and Salditt, T. (2014)
Three-dimensional phase retrieval in propagation-based phase-contrast imaging
Phys. Rev. A, 89(3): 033847, DOI:10.1103/PhysRevA.89.033847

Wilke, R. N., Wallentin, J., Osterhoff, M., Pennicard, D., Zozulya, A., Sprung, M. and Salditt, T. (2014)
High-flux ptychographic imaging using the new 55 µm-pixel detector `Lambda' based on the Medipix3 readout chip
Acta Crystallogr. Sect. A Found. Adv.International Union of Crystallography,, 70(6): 552-562, DOI:10.1107/S2053273314014545

Bartels, M., Hernandez, V. H., Krenkel, M., Moser, T. and Salditt, T. (2013)
Phase contrast tomography of the mouse cochlea at microfocus x-ray sources
Appl. Phys. Lett., 103(8): 083703, DOI:10.1063/1.4818737

Giewekemeyer, K., Wilke, R. N., Osterhoff, M., Bartels, M., Kalbfleisch, S. and Salditt, T. (2013)
Versatility of a hard X-ray Kirkpatrick-Baez focus characterized by ptychography.
J. Synchrotron Radiat., 20(Pt 3): 490-7, DOI:10.1107/S0909049513005372

Krenkel, M., Bartels, M. and Salditt, T. (2013)
Transport of intensity phase reconstruction to solve the twin image problem in holographic x-ray imaging.
Opt. Express, 21(2): 2220-35

Osterhoff, M., Morawe, C., Ferrero, C. and Guigay, J.-P. (2013)
Optimized x-ray multilayer mirrors for single nanometer focusing
Opt. Lett., 38(23): 5126-9

Robisch, A. and Salditt, T. (2013)
Phase retrieval for object and probe using a series of defocus near-field images
Opt. Express, 21(20): 23345-23357, DOI:10.1364/OE.21.023345

Wilke, R. N., Vassholz, M. and Salditt, T. (2013)
Semi-transparent central stop in high-resolution X-ray ptychography using Kirkpatrick-Baez focusing
Acta Crystallogr. Sect. A Found. Crystallogr.International Union of Crystallography,, 69(5): 490-497, DOI:10.1107/S0108767313019612

Bartels, M., Priebe, M., Wilke, R. N., Krüger, S. P., Giewekemeyer, K., Kalbfleisch, S., Olendrowitz, C., Sprung, M. and Salditt, T. (2012)
Low-dose three-dimensional hard x-ray imaging of bacterial cells
Opt. Nanoscopyopen access,, 1(1): 10, DOI:10.1186/2192-2853-1-10

Beerlink, A., Thutupalli, S., Mell, M., Bartels, M., Cloetens, P., Herminghaus, S. and Salditt, T. (2012)
X-Ray propagation imaging of a lipid bilayer in solution
Soft Matter, 8(17): 4595, DOI:10.1039/c2sm00032f

Krüger, S. P., Neubauer, H., Bartels, M., Kalbfleisch, S., Giewekemeyer, K., Wilbrandt, P. J., Sprung, M. and Salditt, T. (2012)
Sub-10 nm beam confinement by X-ray waveguides: design, fabrication and characterization of optical properties.
J. Synchrotron Radiat., 19(Pt 2): 227-36, DOI:10.1107/S0909049511051983

Olendrowitz, C., Bartels, M., Krenkel, M., Beerlink, A., Mokso, R., Sprung, M. and Salditt, T. (2012)
Phase-contrast x-ray imaging and tomography of the nematode Caenorhabditis elegans.
Phys. Med. Biol., 57(16): 5309-23, DOI:10.1088/0031-9155/57/16/5309

Osterhoff, M., Morawe, C., Ferrero, C. and Guigay, J.-P. (2012)
Wave-optical theory of nanofocusing x-ray multilayer mirrors.
Opt. Lett., 37(17): 3705-7

Wilke, R. N., Priebe, M., Bartels, M., Giewekemeyer, K., Diaz, A., Karvinen, P. and Salditt, T. (2012)
Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction
Opt. Express, 20(17): 19232-54

Giewekemeyer, K., Krüger, S. P., Kalbfleisch, S., Bartels, M., Beta, C. and Salditt, T. (2011)
X-ray propagation microscopy of biological cells using waveguides as a quasipoint source
Phys. Rev. A, 83(2): 023804, DOI:10.1103/PhysRevA.83.023804

Osterhoff, M. and Salditt, T. (2011)
Partially coherent x-ray beam simulations: mirrors and more
Adv. Comput. Methods X-Ray Opt. IISPIE,(1): 81410C-81410C-10, DOI:10.1117/12.893003

Osterhoff, M. and Salditt, T. (2011)
Coherence filtering of x-ray waveguides: analytical and numerical approach
New J. Phys.open access,, 13(10): 103026, DOI:10.1088/1367-2630/13/10/103026

Salditt, T., Kalbfleisch, S., Osterhoff, M., Krüger, S. P., Bartels, M., Giewekemeyer, K., Neubauer, H. and Sprung, M. (2011)
Partially coherent nano-focused x-ray radiation characterized by Talbot interferometry
Opt. Express, 19(10): 9656-75

Sedlmair, J., Gleber, S., Öztürk, S., Pfohl, T., Guttmann, P., Wirick, S. and Thieme, J. (2011)
X-Ray Spectromicroscopy of Biomolecular Matter and Soils

Giewekemeyer, K., Neubauer, H., Kalbfleisch, S., Krüger, S. P. and Salditt, T. (2010)
Holographic and diffractive x-ray imaging using waveguides as quasi-point sources
New Journal of Physics, 12(3): 035008

Giewekemeyer, K., Thibault, P., Kalbfleisch, S., Beerlink, A., Kewish, C. M., Dierolf, M., Pfeiffer, F. and Salditt, T. (2010)
Quantitative biological imaging by ptychographic x-ray diffraction microscopy.
Proc. Natl. Acad. Sci. U. S. A., 107(2): 529-34, DOI:10.1073/pnas.0905846107

Krüger, S. P., Giewekemeyer, K., Kalbfleisch, S., Bartels, M., Neubauer, H. and Salditt, T. (2010)
Sub-15 nm beam confinement by twocrossed x-ray waveguides
Opt. ExpressOSA,, 18(13): 13492-13501

Priebe, M., Kalbfleisch, S., Tolkiehn, M., Köster, S., Abel, B., Davies, R. J. and Salditt, T. (2010)
Orientation of biomolecular assemblies in a microfluidic jet
New J. Phys.open access,, 12(4): 043056, DOI:10.1088/1367-2630/12/4/043056

Beerlink, A., Mell, M., Tolkiehn, M. and Salditt, T. (2009)
Hard x-ray phase contrast imaging of black lipid membranes
Applied Physics Letters, 95: 203703, DOI:10.1063/1.3263946

Enders, B., Giewekemeyer, K., Kurz, T., Podorov, S. and Salditt, T. (2009)
Non-iterative coherent diffractive imaging using a phase-shifting reference frame
New Journal of Physics, 11(4): 043021-

Giewekemeyer, K., Hantke, M., Beta, C., Tucoulou, R. and Salditt, T. (2009)
Fluorescence imaging of Dictyostelium discoideum with a hard X-ray nanoprobe
Journal of Physics: Conference Series, 186(1): 012086

Salditt, T., Giewekemeyer, K., Fuhse, C., Kruger, S. P., Tucoulou, R. and Cloetens, P. (2009)
Projection phase contrast microscopy with a hard x-ray nanofocused beam: Defocus and contrast transfer
Phys. Rev. BAPS,, 79(18): 184112-6

Beerlink, A., Wilbrandt, P. J., Ziegler, E., Carbone, D., Metzger, T. H. and Salditt, T. (2008)
X-ray structure analysis of free-standing lipid membranes facilitated by micromachined apertures
Langmuir, 24: 4952-4958, DOI:10.1021/la703704x

Salditt, T., Kruger, S. P., Fuhse, C. and Bahtz, C. (2008)
High-Transmission Planar X-Ray Waveguides
Phys. Rev. Lett.APS,, 100(18): 184801-4