C10 - Coherent x-ray imaging of cells with femtosecond x-ray pulses

Coherent x-ray imaging of cells with femtosecond x-ray pulses
The aim of this project is the label-free imaging of cells at high resolution and high throughput. To reach this goal partial-coherent synchrotron radiation and free electron laser (FEL) pulses will be employed. We will develop microfluidic devices, which guarantee a highly controlled sample environment to bring the sample into the x-ray beam. The combination of different contrast methods and adapted data analysis will lead to a wealth of relevant information about the studied systems.

Members of this project:

Prof. Dr. Sarah Köster
Prof. Dr. Tim Salditt
MSc. Malte Vaßholz
MSc. Chiara Cassini

Associated members of this project:


Wittmeier, A., Cassini, C., Hemonnot, C., Weinhausen, B., Bernhardt, M., Salditt, T. and Köster, S. (2018)
Scanning Small-Angle-X-Ray Scattering for Imaging Biological Cells
Microsc. Microanal., 24 (Suppl 2): 332-334, DOI:10.1017/S1431927618013983

Jahn, T., Wilke, R., Chushkin, Y. and Salditt, T. (2017)
How many photons are needed to reconstruct random objects in coherent X-ray diffractive imaging?
Acta Cryst., 73: 2053-2733, DOI:doi.org/10.1107/S20532733160

Hemonnot, C. J., Reinhardt, J., Saldanha, O., Patommel, J., Graceffa, R., Weinhausen, B., Burghammer, M., Schroer, C. G. and Köster, S. (2016)
X-Rays Reveal the Internal Structure of Keratin Bundles in Whole Cells
ACS Nano, 10(3): 3553-3561, DOI:10.1021/acsnano.5b07871

Block, J., Schroeder, V., Pawelzyk, P., Willenbacher, N. and Köster, S. (2015)
Physical properties of cytoplasmic intermediate filaments
ELSEVIER, 1853: 3053, DOI:doi:10.1016/j.bbamcr.2015.05.009

Piazza, V., Weinhausen, B., Diaz, A., Dammann, C., Maurer, C., Reynolds, M., Burghammer, M. and Köster, S. (2014)
Revealing the Structure of Stereociliary Actin by X-Ray Nano-Imaging
ACS Nano, DOI:10.1021/nn5041526

Weinhausen, B., Saldanha, O., Wilke, R. N., Dammann, C., Priebe, M., Burghammer, M., Sprung, M. and Köster, S. (2014)
Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments
Phys. Rev. Lett., 112(8): 088102, DOI:10.1103/PhysRevLett.112.088102

Wilke, R. N., Quéric, N., Hoppert, M., Heller, C., Schropp, A., Schroer, C., Burghammer, M., Salditt, T. and Reitner, J. (2014)
Scanning Hard X-ray Microscopy Imaging Modalities for Geobiological Samples
Geomicrobiology Journal, accepted for publication, DOI:10.1107/S0108767313019612

Wilke, R. N., Wallentin, J., Osterhoff, M., Pennicard, D., Zozulya, A., Sprung, M. and Salditt, T. (2014)
High-flux ptychographic imaging using the new 55 µm-pixel detector `Lambda' based on the Medipix3 readout chip
Acta Crystallogr. Sect. A Found. Adv.International Union of Crystallography,, 70(6): 552-562, DOI:10.1107/S2053273314014545

Giewekemeyer, K., Wilke, R. N., Osterhoff, M., Bartels, M., Kalbfleisch, S. and Salditt, T. (2013)
Versatility of a hard X-ray Kirkpatrick-Baez focus characterized by ptychography.
J. Synchrotron Radiat., 20(Pt 3): 490-7, DOI:10.1107/S0909049513005372

Mai, D. D., Hallmann, J., Treusch, R., Singer, A., Beckers, M., Gorniak, T., Senkbeil, T., Dronyak, R., Gulden, J., Yefanov, O. M., Rosenhahn, A., Mancuso, A. P., Vartanyants, I. A. and Salditt, T. (2013)
Single pulse coherence measurements in the water window at the free-electron laser FLASH
Opt. Express, 21(11): 13005-13017, DOI:10.1364/OE.21.013005

Weinhausen, B. and Köster, S. (2013)
Microfluidic devices for X-ray studies on hydrated cells.
Lab Chip, 13(2): 212-5, DOI:10.1039/c2lc41014a

Wilke, R. N., Vassholz, M. and Salditt, T. (2013)
Semi-transparent central stop in high-resolution X-ray ptychography using Kirkpatrick-Baez focusing
Acta Crystallogr. Sect. A Found. Crystallogr.International Union of Crystallography,, 69(5): 490-497, DOI:10.1107/S0108767313019612

Köster, S. and Pfohl, T. (2012)
x-ray studies of biological matter in microfluidic environments
Mod. Phys. Lett. B, 26(26): 1230018, DOI:10.1142/S0217984912300189

Priebe, M., Kalbfleisch, S., Tolkiehn, M., Köster, S., Abel, B., Davies, R. J. and Salditt, T. (2010)
Orientation of biomolecular assemblies in a microfluidic jet
New J. Phys.open access,, 12(4): 043056, DOI:10.1088/1367-2630/12/4/043056

Weinhausen, B., Nolting, J.-F., Olendrowitz, C., Langfahl-Klabes, J., Reynolds, M., Salditt, T. and Köster, S. (2012)
X-ray nano-diffraction on cytoskeletal networks
New J. Phys.open access,, 14(8): 085013, DOI:10.1088/1367-2630/14/8/085013